Linked Life Data

20960116

Source:http://linkedlifedata.com/resource/pubmed/id/20960116

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-10-20
pubmed:abstractText
We describe methods to isolate highly active oxygen-evolving photosystem II (PSII) membranes and core complexes from higher plants, and to purify subunits of the oxygen-evolving complex (OEC). The membrane samples used as the material for various in vitro studies of PSII are prepared by solubilizing thylakoid membranes with the nonionic detergent Triton X-100, and the core complexes are prepared by further solubilization of the PSII membranes with n-dodecyl-?-D-maltoside (?-DDM). The OEC subunit proteins are dissociated from the PSII-enriched membranes by alkaline or salt treatment, and are then purified by ion-exchange chromatography using an automated high performance liquid chromatography system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
684
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-10
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants.
pubmed:affiliation
Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
pubmed:publicationType
Journal Article