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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2011-2-25
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pubmed:abstractText |
CyaY is the bacterial homolog of frataxin, proposed to be involved in the assembly of iron-sulfur clusters. While, the physiological iron donor for the iron-sulfur clusters assembly remains controversial. In this study, the gene of CyaY from Acidithiobacillus ferrooxidans was cloned and expressed in Escherichia coli, the protein was purified by one-step affinity chromatography to homogeneity. The CyaY protein can bind ferric iron and serve as an iron donor for the biogenesis of iron-sulfur clusters on the scaffold protein IscU in the presence of IscS and L-cysteine in vitro.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1432-0991
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
733-8
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pubmed:meshHeading |
pubmed-meshheading:20957368-Acidithiobacillus,
pubmed-meshheading:20957368-Amino Acid Sequence,
pubmed-meshheading:20957368-Bacterial Proteins,
pubmed-meshheading:20957368-Chromatography, Affinity,
pubmed-meshheading:20957368-Cloning, Molecular,
pubmed-meshheading:20957368-Escherichia coli,
pubmed-meshheading:20957368-Ferric Compounds,
pubmed-meshheading:20957368-Iron,
pubmed-meshheading:20957368-Models, Molecular,
pubmed-meshheading:20957368-Molecular Chaperones,
pubmed-meshheading:20957368-Molecular Sequence Data,
pubmed-meshheading:20957368-Protein Binding,
pubmed-meshheading:20957368-Recombinant Proteins,
pubmed-meshheading:20957368-Sequence Homology, Amino Acid
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pubmed:year |
2011
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pubmed:articleTitle |
Expression, purification, and characterization of an iron chaperon protein CyaY from Acidithiobacillus ferrooxidans.
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pubmed:affiliation |
Department of Bioengineering, School of Resources Processing and Bioengineering, Central South University, Changsha, 410083, Hunan, People's Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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