Linked Life Data

20956944

Source:http://linkedlifedata.com/resource/pubmed/id/20956944

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2011-2-17
pubmed:abstractText
It has been reported that the miR-106b?25 cluster, a paralog of the miR-17?92 cluster, possesses oncogenic activities. However, the precise role of each microRNA (miRNA) in the miR-106b?25 cluster is not yet known. In this study, we examined the function of miR-93, one of the microRNAs within the miR-106b?25 cluster, in angiogenesis and tumor formation. We found that miR-93 enhanced cell survival, promoted sphere formation and augmented tumor growth. Most strikingly, when miR-93-overexpressing U87 cells were co-cultured with endothelial cells, they supported endothelial cell spreading, growth, migration and tube formation. In vivo studies revealed that miR-93-expressing cells induced blood vessel formation, allowing blood vessels to extend to tumor tissues in high densities. Angiogenesis promoted by miR-93 in return facilitated cell survival, resulting in enhanced tumor growth. We further showed that integrin-?8 is a target of miR-93. Higher levels of integrin-?8 are associated with cell death in tumor mass and in human glioblastoma. Silencing of integrin-?8 expression using small interfering RNA promoted cell proliferation, whereas ectopic expression of integrin-?8 decreased cell growth. These findings showed that miR-93 promotes tumor growth and angiogenesis by suppressing, at least in part, integrin-?8 expression. Our results suggest that inhibition of miR-93 function may be a feasible approach to suppress angiogenesis and tumor growth.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1476-5594
pubmed:author
pubmed:issnType
Electronic
pubmed:day
17
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
806-21
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
MicroRNA miR-93 promotes tumor growth and angiogenesis by targeting integrin-?8.
pubmed:affiliation
Sunnybrook Research Institute, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't