Source:http://linkedlifedata.com/resource/pubmed/id/20955343
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2010-12-24
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pubmed:abstractText |
Engineered skin substitutes (ESSs) comprising both keratinocytes and fibroblasts can afford many advantages over the use of autologous keratinocyte grafts for the treatment of full-thickness and partial-thickness burns. In this study, we investigated the efficacy of a novel ESS containing both genetically altered fibroblasts that express the immunosuppressive factor indoleamine 2,3-dioxygenase (IDO) and primary keratinocytes from a nonautologous source to confer immune protection of xenogeneic cells cultured in a bilayer ESS. The results show that engraftment of IDO expressing skin substitutes on the back of rats significantly improves healing progression over 7 days compared with both nontreated and non-IDO-expressing skin substitutes (p<0.001). Immuno-staining of CD3 and CD31 suggests that IDO-expressing skin substitutes significantly suppress T cell infiltration (p<0.001) and improve neovascularization by four-fold (12.6±1.2 vs. 3.0±1.0 vessel-like structure/high power field), respectively. In conclusion, we found that IDO expression can improve the efficacy of nonautologous ESS for the purpose of wound healing by mitigating T-cell infiltration as well as promoting vascularization of the graft.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
1524-475X
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pubmed:author | |
pubmed:copyrightInfo |
© 2010 by the Wound Healing Society.
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pubmed:issnType |
Electronic
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
614-23
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pubmed:meshHeading |
pubmed-meshheading:20955343-Adenoviridae,
pubmed-meshheading:20955343-Animals,
pubmed-meshheading:20955343-Cell Proliferation,
pubmed-meshheading:20955343-Cells, Cultured,
pubmed-meshheading:20955343-Fibroblasts,
pubmed-meshheading:20955343-Genetic Vectors,
pubmed-meshheading:20955343-Graft Rejection,
pubmed-meshheading:20955343-Immunosuppressive Agents,
pubmed-meshheading:20955343-Indoleamine-Pyrrole 2,3,-Dioxygenase,
pubmed-meshheading:20955343-Neovascularization, Physiologic,
pubmed-meshheading:20955343-Rats,
pubmed-meshheading:20955343-Rats, Sprague-Dawley,
pubmed-meshheading:20955343-Skin, Artificial,
pubmed-meshheading:20955343-T-Lymphocytes,
pubmed-meshheading:20955343-Tissue Engineering,
pubmed-meshheading:20955343-Transfection,
pubmed-meshheading:20955343-Wound Healing
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pubmed:articleTitle |
Local expression of indoleamine 2,3-dioxygenase suppresses T-cell-mediated rejection of an engineered bilayer skin substitute.
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pubmed:affiliation |
Burn and Wound Healing Research Laboratory, Department of Surgery, Immunity and Infection Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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