Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-11-10
pubmed:abstractText
?(3)-Adrenergic receptor agonists are currently under clinical development for the treatment of overactive bladder, a condition that is prevalent in postmenopausal women. These agents purportedly relax bladder smooth muscle through a direct action at the myocyte ?(3)-receptor. The aim of this study was to examine the expression of the individual beta-adrenergic receptors in full thickness sections from ageing human female bladder. We obtained a series of rabbit polyclonal antibodies generated against each of the three ?-adrenergic receptors, and validated their receptor specificity in CHOK1 cells expressing each of the individual receptors. Immunostaining for ?(1), ?(2), and ?(3) were each more prominent in the urothelium than in the detrusor, with all receptors expressed in the same cell types, indicating co-expression of all three receptors throughout the urothelium in addition to the detrusor. Staining of all receptors was also observed in suburothelial myofibroblast-like cells, intramural ganglion cells, and in Schwann cells of intramural nerves. The ?(3)-receptor in the human urothelium appears to be functional, as two different selective ?(3)-receptor agonists, TAK677 and BRL37344, stimulate cAMP formation in URO tsa cells. Densitometry analysis indicates a persistent expression of all receptors throughout the bladder with increasing age, with the exception of the ?(2)-receptor in the urothelium of the trigone, which appears to decrease slightly in older women. These data indicate that ?(3)-receptor expression is maintained with age, but may function in concert with other ?-receptors. Activation of the myocyte receptor may be influenced by action on non-myocyte structures including the intramural ganglion cells and myofibroblasts.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-10027879, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-10188996, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-10455334, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-10830475, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-10945848, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-11125417, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-11508324, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-11564615, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-11786488, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-12015738, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-12223560, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-12853849, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-14570612, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-15013649, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-15123695, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-15621221, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-15855736, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-16091148, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-16465187, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-16736152, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-17028849, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-17211601, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-17407521, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-17445682, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-17631141, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-18311486, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-18490916, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-18820136, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-19156400, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-19172248, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-19198807, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-19374847, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-19429463, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-19515967, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-1967915, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-3727194, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-5014101, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-7715014, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-9228673, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-9647486, http://linkedlifedata.com/resource/pubmed/commentcorrection/20953633-9915482
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1432-0878
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
342
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
295-306
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed-meshheading:20953633-Adrenergic beta-Agonists, pubmed-meshheading:20953633-Adult, pubmed-meshheading:20953633-Age Factors, pubmed-meshheading:20953633-Aged, pubmed-meshheading:20953633-Aged, 80 and over, pubmed-meshheading:20953633-Animals, pubmed-meshheading:20953633-CHO Cells, pubmed-meshheading:20953633-Cricetinae, pubmed-meshheading:20953633-Cricetulus, pubmed-meshheading:20953633-Cyclic AMP, pubmed-meshheading:20953633-Ethanolamines, pubmed-meshheading:20953633-Female, pubmed-meshheading:20953633-Humans, pubmed-meshheading:20953633-Middle Aged, pubmed-meshheading:20953633-Myocytes, Smooth Muscle, pubmed-meshheading:20953633-Myofibroblasts, pubmed-meshheading:20953633-Receptors, Adrenergic, beta, pubmed-meshheading:20953633-Schwann Cells, pubmed-meshheading:20953633-Urinary Bladder, pubmed-meshheading:20953633-Urothelium
pubmed:year
2010
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