Linked Life Data

2095324

Source:http://linkedlifedata.com/resource/pubmed/id/2095324

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1991-7-9
pubmed:abstractText
The effect of retinoic acid on DNA synthesis and cell morphology was studied using corneal stromal fibroblasts in culture. All-trans retinoic acid induces an increase in DNA synthesis after 24 hours of exposure. Autoradiographic studies of 3H-thymidine incorporation into corneal stromal cells exposed to 10(-6) M retinoic acid for 24 hours showed an increase in labeling which ranged from 19.2% to 67.6% over control cultures. Scintillation analysis of labeled cultures also showed an increase in incorporation of 3H-thymidine into cells treated with 10(-6) M retinoic acid, with increases ranging from 21.8% to 114.7% above control cultures. Exposure of cultured corneal stromal cells to 10(-6) M retinoic acid resulted in a dramatic change in cell morphology such that they changed from spindle-shaped to round, flattened cells which were epithelioid in appearance. These data demonstrate that biological activity of retinoic acid in stromal fibroblasts and imply a role for vitamin A in maintenance of stroma structure and function.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0271-3683
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1121-5
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
The effect of retinoic acid on thymidine incorporation and morphology of corneal stromal fibroblasts.
pubmed:affiliation
Department of Ophthalmology, Medical College of Wisconsin, Milwaukee.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.