Linked Life Data

20949361

Source:http://linkedlifedata.com/resource/pubmed/id/20949361

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-11-10
pubmed:abstractText
Non-thyroidal illness is characterized by low tri-iodothyronine (T3) serum level under acute-phase conditions. We studied hepatic gene expression of the newly identified thyroid hormone receptor (TR) cofactor DOR/TP53INP2 together with TRs in a rat model of aseptic abscesses induced by injecting intramuscular turpentine-oil into each hind limb. A fast (4-6 h) decrease in the serum level of free thyroxine and free T3 was observed. By immunohistology, abundant DOR protein expression was detected in the nuclei of hepatocytes and ED-1(+) (mononuclear phagocytes), CK-19(+) (biliary cells), and SMA(+) (mesenchymal cells of the portal tract) cells. DOR signal was reduced with a minimum at 6-12 h after the acute-phase reaction (APR). Immunohistology also showed a similar pattern of protein expression in TR?1 but without a significant change during APR. Transcripts specific for DOR, nuclear receptor co-repressor 1 (NCoR-1), and TR?1 were down-regulated with a minimum at 6-12 h, whereas expression for TR?1 and TR?2 was slightly and significantly up-regulated, respectively, with a maximum at 24 h after APR was initiated. In cultured hepatocytes, acute-phase cytokines interleukin-1? (IL-1?) and IL-6 down-regulated DOR and TR?1 at the mRNA level. Moreover, gene expression of DOR and TRs (TR?1, TR?2, and TR?1) was up-regulated in hepatocytes by adding T3 to the culture medium; this up-regulation was almost completely blocked by treating the cells with IL-6. Thus, TR?1, NCoR-1, and the recently identified DOR/TP53INP2 are abundantly expressed and down-regulated in liver cells during APR. Their down-regulation is attributable to the decreased serum level of thyroid hormones and most probably also to the direct action of the main acute-phase cytokines.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1432-0878
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
342
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
261-72
pubmed:meshHeading
pubmed-meshheading:20949361-Acute-Phase Reaction, pubmed-meshheading:20949361-Animals, pubmed-meshheading:20949361-Cells, Cultured, pubmed-meshheading:20949361-Disease Models, Animal, pubmed-meshheading:20949361-Drug Therapy, Combination, pubmed-meshheading:20949361-Gene Expression, pubmed-meshheading:20949361-Hepatocytes, pubmed-meshheading:20949361-Interleukin-6, pubmed-meshheading:20949361-Liver, pubmed-meshheading:20949361-Male, pubmed-meshheading:20949361-Muscle Proteins, pubmed-meshheading:20949361-Nuclear Receptor Co-Repressor 1, pubmed-meshheading:20949361-RNA, Messenger, pubmed-meshheading:20949361-Rats, pubmed-meshheading:20949361-Rats, Wistar, pubmed-meshheading:20949361-Receptors, Thyroid Hormone, pubmed-meshheading:20949361-Thyroxine, pubmed-meshheading:20949361-Triiodothyronine, pubmed-meshheading:20949361-Turpentine, pubmed-meshheading:20949361-Up-Regulation
pubmed:year
2010
pubmed:articleTitle
Changes in gene expression of DOR and other thyroid hormone receptors in rat liver during acute-phase response.
pubmed:affiliation
Division of Gastroenterology and Endocrinology, Department of Internal Medicine, University Medical Center, Georg August University, Göttingen, Germany. i.malik@med.uni-goettingen.de
pubmed:publicationType
Journal Article