Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2010-10-14
pubmed:abstractText
Twenty five compounds including ten triterpenes (1-3, 5-11), six flavonoids (12-15, 24, 25), five lignans (17, 18, 21-23), two butenyl clohexnone glycosides (19-20), one fructofuranoside (16) and one fatty acid (4) were isolated from the roots of Ulmus davidiana var. japonica. The structures of those compounds were identified by comparing their physicochemical and spectral data with those of published in literatures. All the compounds were evaluated for DNA topoisomerase inhibitory activities and cytotoxicities. Among the purified compounds, 4 and 19 showed more potent inhibitory acitivities (IC(50): 39 and 19 ?M, respectively) than camptothecin, as the positive control (IC(50): 46 ?M) against topoisomerase I. Compounds, 4, 10, 12, 19, 24 and 25 showed strong inhibitory activities toward DNA topoisomerase II (IC(50): 0.1, 0.52, 0.47, 0.42, 0.17 ?M and 17 nM, respectively), which were more potent than that of etoposide as positive control (IC(50): 20 ?M). In A549 cell line, 5 and 6 showed cytotoxicities (IC(50): 4 ?M and 3 ?M, respectively, with IC(50) of camptothecin as positive control: 10.3 ?M). In the HepG2 cell line, 3, 5 and 7 showed cytotoxicity (IC(50): 4, 3 and 4 ?M, respectively, with IC(50) of camptothecin: 0.3 ?M). Compounds 6, 12 and 23 showed cytotoxicities in the HT-29 cell line (IC(50): 19, 19 and 15 ?M, respectively, with IC(50) of camptothecin: 2 ?M).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0253-6269
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1307-15
pubmed:meshHeading
pubmed-meshheading:20945128-Antineoplastic Agents, Phytogenic, pubmed-meshheading:20945128-Cell Line, Tumor, pubmed-meshheading:20945128-Cyclohexanones, pubmed-meshheading:20945128-Drug Discovery, pubmed-meshheading:20945128-Drug Screening Assays, Antitumor, pubmed-meshheading:20945128-Eicosanoic Acids, pubmed-meshheading:20945128-Flavonoids, pubmed-meshheading:20945128-Glucosides, pubmed-meshheading:20945128-Humans, pubmed-meshheading:20945128-Inhibitory Concentration 50, pubmed-meshheading:20945128-Medicine, Korean Traditional, pubmed-meshheading:20945128-Molecular Structure, pubmed-meshheading:20945128-Neoplasms, pubmed-meshheading:20945128-Plant Bark, pubmed-meshheading:20945128-Plant Extracts, pubmed-meshheading:20945128-Plant Roots, pubmed-meshheading:20945128-Spectrometry, Mass, Fast Atom Bombardment, pubmed-meshheading:20945128-Topoisomerase I Inhibitors, pubmed-meshheading:20945128-Topoisomerase II Inhibitors, pubmed-meshheading:20945128-Triterpenes, pubmed-meshheading:20945128-Ulmus
pubmed:year
2010
pubmed:articleTitle
Inhibition of DNA topoisomerases I and II and cytotoxicity of compounds from Ulmus davidiana var. japonica.
pubmed:affiliation
College of Pharmacy, Yeungnam University, Gyongsan, 712-749, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't