Linked Life Data

20937868

Source:http://linkedlifedata.com/resource/pubmed/id/20937868

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
43
pubmed:dateCreated
2010-10-27
pubmed:abstractText
Most antigenic peptides presented by MHC class I molecules result from the degradation of intracellular proteins by the proteasome. In lymphoid tissues and cells exposed to IFN?, the standard proteasome is replaced by the immunoproteasome, in which all of the standard catalytic subunits ?1, ?2, and ?5 are replaced by their inducible counterparts ?1i, ?2i, and ?5i, which have different cleavage specificities. The immunoproteasome thereby shapes the repertoire of antigenic peptides. The existence of additional forms of proteasomes bearing a mixed assortment of standard and inducible catalytic subunits has been suggested. Using a new set of unique subunit-specific antibodies, we have now isolated, quantified, and characterized human proteasomes that are intermediate between the standard proteasome and the immunoproteasome. They contain only one (?5i) or two (?1i and ?5i) of the three inducible catalytic subunits of the immunoproteasome. These intermediate proteasomes represent between one-third and one-half of the proteasome content of human liver, colon, small intestine, and kidney. They are also present in human tumor cells and dendritic cells. We identified two tumor antigens of clinical interest that are processed exclusively either by intermediate proteasomes ?5i (MAGE-A3(271-279)) or by intermediate proteasomes ?1i-?5i (MAGE-A10(254-262)). The existence of these intermediate proteasomes broadens the repertoire of antigens presented to CD8 T cells and implies that the antigens presented by a given cell depend on their proteasome content.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1091-6490
pubmed:author
pubmed:issnType
Electronic
pubmed:day
26
pubmed:volume
107
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
18599-604
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Two abundant proteasome subtypes that uniquely process some antigens presented by HLA class I molecules.
pubmed:affiliation
Ludwig Institute for Cancer Research, Brussels Branch, 1200 Brussels, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't