Source:http://linkedlifedata.com/resource/pubmed/id/20935649
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
2010-10-29
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pubmed:abstractText |
Non-cell-autonomous proteins are incorporated into cells that form tight contacts or are invaded by bacteria, but identifying the full repertoire of transferred proteins has been a challenge. Here we introduce a quantitative proteomics approach to sort out non-cell-autonomous proteins synthesized by other cells or intracellular pathogens. Our approach combines stable-isotope labeling of amino acids in cell culture (SILAC), high-purity cell sorting and bioinformatics analysis to identify the repertoire of relevant non-cell-autonomous proteins. This 'trans-SILAC' method allowed us to discover many proteins transferred from human B to natural killer cells and to measure biosynthesis rates of Salmonella enterica proteins in infected human cells. Trans-SILAC should be a useful method to examine protein exchange between different cells of multicellular organisms or pathogen and host.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1548-7105
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
923-7
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pubmed:meshHeading |
pubmed-meshheading:20935649-Antigens, CD86,
pubmed-meshheading:20935649-Bacterial Proteins,
pubmed-meshheading:20935649-Cell Communication,
pubmed-meshheading:20935649-Cells, Cultured,
pubmed-meshheading:20935649-Humans,
pubmed-meshheading:20935649-Isotope Labeling,
pubmed-meshheading:20935649-Protein Biosynthesis,
pubmed-meshheading:20935649-Protein Transport,
pubmed-meshheading:20935649-Proteome
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pubmed:year |
2010
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pubmed:articleTitle |
Trans-SILAC: sorting out the non-cell-autonomous proteome.
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pubmed:affiliation |
Department of Neurobiology, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
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pubmed:publicationType |
Journal Article
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