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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2011-5-5
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pubmed:abstractText |
The adult human anterior cruciate ligament (ACL) has poor functional healing response. Transforming growth factor (TGF)-?1 enhances the wound repair by stimulating matrix proteins deposition as well as the proliferation and migration of cells. However, the function of the TGF-?1-induced matrix metalloproteinases' (MMPs) activities in the wound healing process is poorly understood. In this study, exogenous MMP-2 is added to mimic the TGF-?1-induced MMP-2 expression. Role of NF-?B pathway is further examined. Our results show that TGF-?1 induces dramatic elevation of MMP-2 activities and the MMP-2/tissue inhibitors of metalloproteinases ratio. Furthermore, the exogenous MMP-2 significantly promoted in vitro wound healing abilities of ACL fibroblasts that are significantly blocked with the addition of its inhibitors. TGF-?1 also increases the proliferation of ACL fibroblasts whereas MMP-2 alone does not, indicating that MMP-2 activities are not involved in the proliferation. TGF-?1-induced MMP-2 activity is inhibited by Bay11-7082 and Bay11-7085 (NF-?B inhibitors). Our results demonstrate that increased TGF-?1 facilitates the ACL healing process by promoting the fibroblasts migration and proliferation. The migration process is mediated by MMP-2 and NF-?B pathway is involved in TGF-?1-mediated MMP-2 release.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-(4-methylphenylsulfonyl)-2-propene...,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/MMP2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nitriles,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfones,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Inhibitor of...,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Inhibitor of...,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1607-8438
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
52
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
218-25
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pubmed:meshHeading |
pubmed-meshheading:20932173-Adult,
pubmed-meshheading:20932173-Anterior Cruciate Ligament,
pubmed-meshheading:20932173-Blotting, Western,
pubmed-meshheading:20932173-Cell Proliferation,
pubmed-meshheading:20932173-Culture Media, Conditioned,
pubmed-meshheading:20932173-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:20932173-Fibroblasts,
pubmed-meshheading:20932173-Humans,
pubmed-meshheading:20932173-Matrix Metalloproteinase 2,
pubmed-meshheading:20932173-Middle Aged,
pubmed-meshheading:20932173-NF-kappa B,
pubmed-meshheading:20932173-Nitriles,
pubmed-meshheading:20932173-Sulfones,
pubmed-meshheading:20932173-Time Factors,
pubmed-meshheading:20932173-Tissue Inhibitor of Metalloproteinase-1,
pubmed-meshheading:20932173-Tissue Inhibitor of Metalloproteinase-2,
pubmed-meshheading:20932173-Transforming Growth Factor beta1,
pubmed-meshheading:20932173-Wound Healing,
pubmed-meshheading:20932173-Young Adult
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pubmed:year |
2011
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pubmed:articleTitle |
TGF-?1 promoted MMP-2 mediated wound healing of anterior cruciate ligament fibroblasts through NF-?B.
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pubmed:affiliation |
Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University , Chongqing , PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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