Linked Life Data

20926142

Source:http://linkedlifedata.com/resource/pubmed/id/20926142

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2011-1-14
pubmed:abstractText
We investigated the expression of the partially duplicated ?7 nAChR subunit gene in three monocytic cell lines: THP-1, U937 and Mono-Mac-6. Qualitative PCR revealed the presence of the classic ?7 gene in these lineages, but real-time PCR showed the exclusive expression of ?7dup. Both mRNA and protein levels were reduced in THP-1 upon LPS challenge, and it was found that transcriptional down-regulation was mediated by a direct mechanism dependent on NF-?B as its specific inhibitor parthenolide prevented the reduction in the ?7dup transcript. Such precise regulation suggests that ?7dup may specifically participate in the inflammatory response of the innate immune system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1872-8421
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
230
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
74-84
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Expression of the ?7 nAChR subunit duplicate form (CHRFAM7A) is down-regulated in the monocytic cell line THP-1 on treatment with LPS.
pubmed:affiliation
Department of Pharmacology, School of Medicine, Università degli Studi di Milano, Milan, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't