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pubmed-article:20921938pubmed:abstractTextThe multifunctional protein p100 is a vital transcriptional regulator that increases gene transcription by forming a physical bridge between promoter-specific transcription factors and the basal transcription machinery. To identify potential signal transduction pathways in which human p100 acts as a coregulator and to find target promoter regions that may interact with p100, we performed a promoter microarray assay called chromatin immunoprecipitation-guided ligation and selection (ChIP-GLAS). From this assay, we determined that a set of promoter fragments, including several factors in the transforming growth factor beta (TGF-?) signaling pathway, exhibited interaction with p100. The ChIP-GLAS data were validated by RT-PCR assessing the mRNA expression of various factors in the TGF-? signaling pathway in cell lines.lld:pubmed
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pubmed-article:20921938pubmed:authorpubmed-author:SzeS HSHlld:pubmed
pubmed-article:20921938pubmed:authorpubmed-author:YangJieJlld:pubmed
pubmed-article:20921938pubmed:authorpubmed-author:XiangJingJlld:pubmed
pubmed-article:20921938pubmed:authorpubmed-author:ZhangXuejunXlld:pubmed
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pubmed-article:20921938pubmed:authorpubmed-author:WangXintingXlld:pubmed
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pubmed-article:20921938pubmed:authorpubmed-author:WangBaoyaBlld:pubmed
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pubmed-article:20921938pubmed:year2011lld:pubmed
pubmed-article:20921938pubmed:articleTitleIdentification of p100 target promoters by chromatin immunoprecipitation-guided ligation and selection (ChIP-GLAS).lld:pubmed
pubmed-article:20921938pubmed:affiliationDepartment of Immunology, Basic Medical College, Tianjin Medical University, Tianjin, China.lld:pubmed
pubmed-article:20921938pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:20921938pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed