Source:http://linkedlifedata.com/resource/pubmed/id/20921938
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2011-1-4
|
| pubmed:abstractText |
The multifunctional protein p100 is a vital transcriptional regulator that increases gene transcription by forming a physical bridge between promoter-specific transcription factors and the basal transcription machinery. To identify potential signal transduction pathways in which human p100 acts as a coregulator and to find target promoter regions that may interact with p100, we performed a promoter microarray assay called chromatin immunoprecipitation-guided ligation and selection (ChIP-GLAS). From this assay, we determined that a set of promoter fragments, including several factors in the transforming growth factor beta (TGF-?) signaling pathway, exhibited interaction with p100. The ChIP-GLAS data were validated by RT-PCR assessing the mRNA expression of various factors in the TGF-? signaling pathway in cell lines.
|
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
2042-0226
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
8
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
88-91
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| pubmed:meshHeading |
pubmed-meshheading:20921938-Binding Sites,
pubmed-meshheading:20921938-Chromatin Immunoprecipitation,
pubmed-meshheading:20921938-Female,
pubmed-meshheading:20921938-Gene Expression Regulation,
pubmed-meshheading:20921938-Genes, Reporter,
pubmed-meshheading:20921938-HeLa Cells,
pubmed-meshheading:20921938-Humans,
pubmed-meshheading:20921938-Nuclear Proteins,
pubmed-meshheading:20921938-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:20921938-Promoter Regions, Genetic,
pubmed-meshheading:20921938-Protein Binding,
pubmed-meshheading:20921938-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:20921938-Signal Transduction,
pubmed-meshheading:20921938-Trans-Activators,
pubmed-meshheading:20921938-Transcription, Genetic,
pubmed-meshheading:20921938-Transcriptional Activation,
pubmed-meshheading:20921938-Transforming Growth Factor beta
|
| pubmed:year |
2011
|
| pubmed:articleTitle |
Identification of p100 target promoters by chromatin immunoprecipitation-guided ligation and selection (ChIP-GLAS).
|
| pubmed:affiliation |
Department of Immunology, Basic Medical College, Tianjin Medical University, Tianjin, China.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|