Linked Life Data

20920471

Source:http://linkedlifedata.com/resource/pubmed/id/20920471

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-11-3
pubmed:abstractText
Because microtubules are the structural elements of cells, it is essential to study the mechanical properties of single microtubules under physiological conditions. Previously, we measured the effect of temperature on the flexural rigidity of a single taxol-stabilized microtubule and found that the flexural rigidity is 2.5×10(-24)Nm(2), independent of temperature in the 20-35°C range. Employing the same technique here, we have measured the flexural rigidity of microtubules polymerized in the presence of guanylyl-(a,b)-methylene-diphosphonate (GMPCPP, the slowly hydrolyzable GTP analogue) and in the presence of GTP only; both of the states were taxol-free. The obtained values were approximately 5-fold (for GMPCPP) and three- to 4-fold (for GTP) greater than those of taxol-stabilized microtubules. Interestingly, rigidity decreased as temperature increased, that is, temperature dependence was only observed in taxol-free microtubules. Length dependence was also observed. These results indicate that the transition of microtubule's rigidity is associated with the tubulin conformation change from a GTP-bound state to a GDP-bound state in the ?/? subunit. We discuss the relationship of the regulation mechanism of the microtubules in the cell body to the changes in rigidity through hydrolysis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1090-2104
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
5
pubmed:volume
402
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
66-9
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Temperature dependence rigidity of non-taxol stabilized single microtubules.
pubmed:affiliation
Department of Neurobiology, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba 260-8670, Japan. kkawaguchi-cib@umin.ac.jp
pubmed:publicationType
Journal Article