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rdf:type |
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lifeskim:mentions |
umls-concept:C0041967,
umls-concept:C0086466,
umls-concept:C0220825,
umls-concept:C0450442,
umls-concept:C0991566,
umls-concept:C1272883,
umls-concept:C1272936,
umls-concept:C1522518,
umls-concept:C1523169,
umls-concept:C1533685,
umls-concept:C1533691
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pubmed:issue |
5-6
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pubmed:dateCreated |
2011-2-28
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pubmed:abstractText |
Growth factor (basic fibroblast growth factor or vascular endothelial growth factor)-immobilized polycaprolactone (PCL)/Pluronic F127 porous beads were prepared as an injectable bulking agent for effective treatment of urinary incontinence. The growth factor-immobilized porous beads may stimulate smooth muscle cell (SMC) differentiation of muscle-derived stem cells or defect tissues around urethra to improve the sphincter function (bioactive therapy) as well as to provide a bulking effect (passive therapy). The porous PCL/F127 beads were fabricated by an isolated particle-melting/melt-molding particulate-leaching method. The growth factors were easily immobilized onto the surfaces of the PCL/F127 porous beads via heparin binding and were continuously released for up to 28 days. Both growth factor-immobilized porous beads had a positive effect for the SMC differentiation of muscle-derived stem cells, as were demonstrated by the analyses of quantitative polymerase chain reactions, Western blot using SMC-specific markers, and immunohistochemical staining. In particular, the basic fibroblast growth factor-immobilized porous beads showed desirable SMC differentiation behavior that can be applied as an injectable bulking agent for the treatment of urinary incontinence.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1937-335X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
655-64
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pubmed:meshHeading |
pubmed-meshheading:20919951-Animals,
pubmed-meshheading:20919951-Biocompatible Materials,
pubmed-meshheading:20919951-Blotting, Western,
pubmed-meshheading:20919951-Cell Differentiation,
pubmed-meshheading:20919951-DNA,
pubmed-meshheading:20919951-Fibroblast Growth Factor 2,
pubmed-meshheading:20919951-Heparin,
pubmed-meshheading:20919951-Immobilized Proteins,
pubmed-meshheading:20919951-Injections,
pubmed-meshheading:20919951-Microscopy, Confocal,
pubmed-meshheading:20919951-Microscopy, Electron, Scanning,
pubmed-meshheading:20919951-Microspheres,
pubmed-meshheading:20919951-Muscles,
pubmed-meshheading:20919951-Myocytes, Smooth Muscle,
pubmed-meshheading:20919951-Poloxamer,
pubmed-meshheading:20919951-Polyesters,
pubmed-meshheading:20919951-Polymerase Chain Reaction,
pubmed-meshheading:20919951-Porosity,
pubmed-meshheading:20919951-Rats,
pubmed-meshheading:20919951-Stem Cells,
pubmed-meshheading:20919951-Urethra,
pubmed-meshheading:20919951-Vascular Endothelial Growth Factor A
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pubmed:year |
2011
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pubmed:articleTitle |
Bioactive porous beads as an injectable urethral bulking agent: their in vitro evaluation on smooth muscle cell differentiation.
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pubmed:affiliation |
Department of Advanced Materials, Hannam University, Yuseong Gu, Daejeon, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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