Linked Life Data

2090659

Source:http://linkedlifedata.com/resource/pubmed/id/2090659

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1991-6-5
pubmed:abstractText
N-Glycosidically bound sialooligosaccharides in a model glycoprotein of serum type (porcine thyroglobulin) were released by pronase digestion, followed by hydrazinolysis. The resulting oligosaccharides were re-N-acetylated and reduced with sodium borohydride. On the other hand, O-glycosidically bound sialooligosaccharides were released from a mucin-type glycoprotein (bovine submaxillary mucin) with alkali in the presence of sodium borohydride. The reduced oligosaccharides thus obtained from both types of glycoproteins were analysed by high-performance liquid chromatography on a column of a latex-type pellicular anion-exchange resin with strong alkali as eluent. These sequential procedures were useful for mapping of oligosaccharides in glycoproteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
523
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
189-200
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Analysis of N- and O-glycosidically bound sialooligosaccharides in glycoproteins by high-performance liquid chromatography with pulsed amperometric detection.
pubmed:affiliation
Faculty of Pharmaceutical Sciences, Kinki University, Higashi-osaka, Japan.
pubmed:publicationType
Journal Article