Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
|
pubmed:dateCreated |
1991-5-30
|
pubmed:abstractText |
The synthesis rate of brain acetylcholine (ACh) was estimated in mice following i.v. administration of [3H]choline (Ch). The measurements were performed 1 min after the tracer injection, using the [3H]ACh/[3H]Ch specific radioactivity ratio as an index of ACh synthesis rate. Endogenous and labeled Ch and ACh were quantified using HPLC methodology. Oxotremorine and physostigmine (0.5 mg/kg, i.p.) increased the steady state concentration of brain ACh by + 130% and 84%, respectively and of Ch by + 60% (oxotremorine); they decreased ACh synthesis by 62 and 55%, respectively. By contrast, scopolamine (0.7 mg/kg, i.p.) decreased the cerebral content of Ch by - 26% and of ACh by - 23% without enhancing the synthesis of ACh. The results show the utility of HPLC methodology in the investigation of ACh turnover.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Choline,
http://linkedlifedata.com/resource/pubmed/chemical/Oxotremorine,
http://linkedlifedata.com/resource/pubmed/chemical/Physostigmine,
http://linkedlifedata.com/resource/pubmed/chemical/Scopolamine Hydrobromide,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
|
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0364-3190
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
15
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1097-100
|
pubmed:dateRevised |
2011-11-17
|
pubmed:meshHeading |
pubmed-meshheading:2089268-Acetylcholine,
pubmed-meshheading:2089268-Animals,
pubmed-meshheading:2089268-Brain,
pubmed-meshheading:2089268-Choline,
pubmed-meshheading:2089268-Chromatography, High Pressure Liquid,
pubmed-meshheading:2089268-Male,
pubmed-meshheading:2089268-Mice,
pubmed-meshheading:2089268-Oxotremorine,
pubmed-meshheading:2089268-Physostigmine,
pubmed-meshheading:2089268-Scopolamine Hydrobromide,
pubmed-meshheading:2089268-Tritium
|
pubmed:year |
1990
|
pubmed:articleTitle |
Effect of oxotremorine, physostigmine, and scopolamine on brain acetylcholine synthesis: a study using HPLC.
|
pubmed:affiliation |
Laboratoire de Pharmacodynamie et de Physiologie Pharmaceutique, Faculté de Pharmacie, Dijon, France.
|
pubmed:publicationType |
Journal Article
|