Source:http://linkedlifedata.com/resource/pubmed/id/20890662
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2011-2-2
|
| pubmed:databankReference | |
| pubmed:abstractText |
Enterotoxigenic Eschoerchia coli (ETEC) F4 is a major cause of diarrhea in neonatal and young pigs. The locus encoding intestinal receptor for ETEC F4ab/ac has been mapped to pig chromosome (SSC) 13q41. We previously assigned mucin 20 (MUC20) to SSC13q41 and considered it a candidate for the ETEC F4ab/ac receptor. In this study, the full-length mRNA sequence of the porcine MUC20 gene was determined, revealing two MUC20 alternative transcripts differing in an 18-bp deletion in the open reading frame (ORF). The longer isoform is 1,927-bp long and contains a 1,572-bp ORF encoding a protein of 523 amino acids. RT-PCR analysis showed that MUC20 mRNA was expressed most highly in the kidney, prostate, epididymis and bladder. Seven single nucleotide polymorphisms in MUC20 were identified in founder animals of a White Duroc × Erhualian cross, and the entire cross population (n = 842) was genotyped for two MUC20 polymorphisms: g.191C>T in intron 5 and c.1600C>T in exon 6. The transmission disequilibrium test (TDT) results revealed that both polymorphisms were significantly associated with in vitro ETEC F4ab/ac adhesion phenotypes in the intercross population, and the most significant association was found between g.191C>T and F4ac adhesion phenotype (P = 1.02 × 10(-9)).
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1573-4978
|
| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:volume |
38
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1593-601
|
| pubmed:meshHeading |
pubmed-meshheading:20890662-Alleles,
pubmed-meshheading:20890662-Alternative Splicing,
pubmed-meshheading:20890662-Animals,
pubmed-meshheading:20890662-Crosses, Genetic,
pubmed-meshheading:20890662-DNA, Complementary,
pubmed-meshheading:20890662-Enterotoxigenic Escherichia coli,
pubmed-meshheading:20890662-Escherichia coli Infections,
pubmed-meshheading:20890662-Female,
pubmed-meshheading:20890662-Gene Expression Profiling,
pubmed-meshheading:20890662-Genetic Predisposition to Disease,
pubmed-meshheading:20890662-Haplotypes,
pubmed-meshheading:20890662-Linkage Disequilibrium,
pubmed-meshheading:20890662-Male,
pubmed-meshheading:20890662-Molecular Sequence Data,
pubmed-meshheading:20890662-Mucins,
pubmed-meshheading:20890662-Polymorphism, Single Nucleotide,
pubmed-meshheading:20890662-RNA, Messenger,
pubmed-meshheading:20890662-Sus scrofa,
pubmed-meshheading:20890662-Swine,
pubmed-meshheading:20890662-Swine Diseases
|
| pubmed:year |
2011
|
| pubmed:articleTitle |
The porcine MUC20 gene: molecular characterization and its association with susceptibility to enterotoxigenic Escherichia coli F4ab/ac.
|
| pubmed:affiliation |
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045 Nanchang, China.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|