Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1991-5-24
pubmed:abstractText
As methods for human islet isolation improve and clinical trials become widespread, some form of storage will be needed. Cryopreservation would offer many advantages: storage in a tissue bank to provide sufficient islets to transplant to individual recipients; modulation of tissue immunogenicity; purification; and, facilitate shipment from center to center. Since 1976, much work has been done on islet cryopreservation with cooling rates of 0.025 degrees C/min to 75 degrees C/min being reported as optimum. In rats we have found that slow cooling to -40 degrees C with rapid thawing from -196 degrees C gives the highest survival as measured in-vitro and in-vivo. This freeze-thaw protocol also provides viable pancreatic microfragments and pure dog islets which can both induce prolonged normoglycemia. This freezing procedure also gives prolonged survival of islet xenografts and purifies the pancreatic microfragments of the unwanted exocrine contaminants. This method of freezing is now being used for human islets. Low temperature storage of human islets will greatly help the clinical use of islet transplantation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0170-5903
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
72-81
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Islet cryopreservation.
pubmed:affiliation
Department of Surgery, University of Alberta, Edmonton, Canada.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't