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rdf:type |
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lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0030580,
umls-concept:C0036536,
umls-concept:C0036537,
umls-concept:C0039005,
umls-concept:C0086418,
umls-concept:C0109329,
umls-concept:C0348011,
umls-concept:C1517600,
umls-concept:C1704353,
umls-concept:C1705822
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pubmed:issue |
1
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pubmed:dateCreated |
2011-1-6
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pubmed:abstractText |
Previously (Shan et al, 2005), we reported that adenoviral vector-mediated transfer of the human aquaporin-1 (hAQP1) cDNA to minipig parotid glands following irradiation (IR) transiently restored salivary flow to near normal levels. This study evaluated a serotype 2, adeno-associated viral (AAV2) vector for extended correction of IR (single dose; 20?Gy)-induced, parotid salivary hypofunction in minipigs. At 16 weeks following the IR parotid salivary flow decreased by 85-90%. AAV2hAQP1 administration at week 17 transduced only duct cells and resulted in a dose-dependent increase in salivary flow to approximately 35% of pre-IR levels (to approximately 1?ml per 10 min) after 8 weeks (peak response). Administration of a control AAV2 vector or saline was without effect. Little change was observed in clinical chemistry and hematology values after AAV2hAQP1 delivery. Vector-treated animals generated high anti-AAV2 neutralizing antibody titers by week 4 (approximately 1:1600) and significant elevations in salivary (approximately 15%), but not serum, granulocyte macrophage colony-stimulating factor levels. Following vector administration, salivary [Na(+)] was dramatically increased, from approximately 10 to approximately 55?mM (at 4 weeks) and finally to 39 mM (8 weeks). The findings demonstrate that localized delivery of AAV2hAQP1 to IR-damaged parotid glands leads to increased fluid secretion from surviving duct cells, and may be useful in providing extended relief of salivary hypofunction in previously irradiated patients.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20882054-11271485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20882054-12788413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20882054-12799323,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20882054-17726456,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20882054-20224180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20882054-7530838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20882054-7713792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20882054-9096382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20882054-9508048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20882054-9874275
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1476-5462
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pubmed:author |
pubmed-author:AfioneSS,
pubmed-author:BaumB JBJ,
pubmed-author:ChioriniJ AJA,
pubmed-author:GaoRR,
pubmed-author:GoldsmithC MCM,
pubmed-author:HaiBB,
pubmed-author:WangSS,
pubmed-author:XuJJ,
pubmed-author:YauSS,
pubmed-author:ZhangCC,
pubmed-author:ZhengCC,
pubmed-author:ZhouJJ
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pubmed:issnType |
Electronic
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
38-42
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pubmed:dateRevised |
2011-8-1
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pubmed:meshHeading |
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pubmed:year |
2011
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pubmed:articleTitle |
AAV2-mediated transfer of the human aquaporin-1 cDNA restores fluid secretion from irradiated miniature pig parotid glands.
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pubmed:affiliation |
Salivary Gland Disease Center and Molecular Laboratory for Gene Therapy, Capital Medical University School of Stomatology, Beijing, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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