Linked Life Data

20878105

Source:http://linkedlifedata.com/resource/pubmed/id/20878105

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-9-29
pubmed:abstractText
To examine the effect of scutellarin on adhesion and migration of oral squamous cell carcinoma (OSCC), the HSC-4 and SAS human OSCC cells were treated with various concentrations of scutellarin. Scutellarin cytotoxicity was evaluated by MTT assays; migration of tongue cancer cells was assessed by wound healing and Transwell chemotaxis; ?v?6 integrin and E-cadherin expression was assessed by immunofluorescence and reverse transcription polymerase chain reaction. Scutellarin at 75 nM significantly inhibited tongue cancer cell proliferation and at 15 nM, significantly reduced HSC-4 and SAS cell motility by 46.3% and 44%, respectively. Scutellarin inhibited SAS cell adhesion to fibronectin in a dose-dependent manner. However, it had no significant effect on HSC-4 cell adhesion to fibronectin; at the same concentration, HSC-4 cells adhered more strongly to fibronectin than SAS cells. Following treatment with scutellarin, E-cadherin and desmoplakin protein levels were increased, whereas E-cadherin mRNA expression was unchanged; protein levels of ?v?6 integrin were decreased-consistent with the change in ?v?6 integrin mRNA. After a 3 nM scutellarin treatment, levels of desmoplakin in HSC-4 and SAS cells increased by 79.9% and 74.5%, respectively. Scutellarin (3 nM) increased expression of E-cadherin in HSC-4 and SAS cells by 37.9% and 52%, respectively, and decreased the expression of ?v?6 integrin by 45.4% and 47.2%, respectively. This study shows that scutellarin inhibits tumor cell proliferation and migration and regulates cell adhesion in OSCC cells; this may be closely related to up-regulation of E-cadherin and down-regulation of ?v?6 integrin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1791-2431
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1153-60
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Scutellarin inhibits cell migration by regulating production of ?v?6 integrin and E-cadherin in human tongue cancer cells.
pubmed:affiliation
Department of Anatomy, Basic Medical Science College, The Second Clinical College, Harbin Medical University, Harbin 150081, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't