| pubmed-article:20868728 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20868728 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:20868728 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:20868728 | lifeskim:mentions | umls-concept:C0040613 | lld:lifeskim |
| pubmed-article:20868728 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:20868728 | lifeskim:mentions | umls-concept:C1420101 | lld:lifeskim |
| pubmed-article:20868728 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:20868728 | lifeskim:mentions | umls-concept:C1708632 | lld:lifeskim |
| pubmed-article:20868728 | lifeskim:mentions | umls-concept:C1522485 | lld:lifeskim |
| pubmed-article:20868728 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:20868728 | pubmed:dateCreated | 2010-11-1 | lld:pubmed |
| pubmed-article:20868728 | pubmed:abstractText | Aim of this study was to examine the dipeptide transport of ?-Ala-Lys-N(?)-AMCA in the human glioma cell line U373-MG and its potential regulation by diverse hormones and culture media. A mixed glial primary cell culture of the newborn rat served as reference cell system. ?-Ala-Lys-N(?)-AMCA (?-Ala-Lys-N(?)-7-amino-4-methyl-coumarin-3-acetic acid) is a highly specific reporter substrate to investigate the dipeptide transport system PepT2. We were able to demonstrate that U373-MG cells express PepT2-mRNA and translocate ?-Ala-Lys-N(?)-AMCA via PepT2 into the cytoplasm. Previous results demonstrated that ?-Ala-Lys-N(?)-AMCA specifically accumulates in differentiated and dedifferentiated astrocytes but neither in differentiated nor dedifferentiated oligodendrocytes and in neurons. U373-MG cells were incubated with estradiol, testosterone, thyronine, dexamethasone, dibutyryl cyclic adenosine monophosphate and tetradecanoylphorbol acetate in order to detect potential substance-dependent changes in dipeptide uptake. There was no significant increase or decrease of ?-Ala-Lys-N(?)-AMCA-uptake after stimulation. Northern blot analyses confirmed that PepT2-mRNA is expressed in U373-MG and glial cells but showed no regulation of PepT2-mRNA expression in both cell types. Future investigations might offer the opportunity of an anti-tumor therapy with cytotoxic agents linked to a dipeptide-derivative such as ?-Ala-Lys. | lld:pubmed |
| pubmed-article:20868728 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20868728 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20868728 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20868728 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20868728 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20868728 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20868728 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20868728 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20868728 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20868728 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20868728 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:20868728 | pubmed:issn | 1872-7972 | lld:pubmed |
| pubmed-article:20868728 | pubmed:author | pubmed-author:KappertKaiK | lld:pubmed |
| pubmed-article:20868728 | pubmed:author | pubmed-author:ZimmermannMat... | lld:pubmed |
| pubmed-article:20868728 | pubmed:author | pubmed-author:StanAlexandru... | lld:pubmed |
| pubmed-article:20868728 | pubmed:copyrightInfo | Copyright © 2010 Elsevier Ireland Ltd. All rights reserved. | lld:pubmed |
| pubmed-article:20868728 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20868728 | pubmed:day | 17 | lld:pubmed |
| pubmed-article:20868728 | pubmed:volume | 486 | lld:pubmed |
| pubmed-article:20868728 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20868728 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20868728 | pubmed:pagination | 174-8 | lld:pubmed |
| pubmed-article:20868728 | pubmed:meshHeading | pubmed-meshheading:20868728... | lld:pubmed |
| pubmed-article:20868728 | pubmed:meshHeading | pubmed-meshheading:20868728... | lld:pubmed |
| pubmed-article:20868728 | pubmed:meshHeading | pubmed-meshheading:20868728... | lld:pubmed |
| pubmed-article:20868728 | pubmed:meshHeading | pubmed-meshheading:20868728... | lld:pubmed |
| pubmed-article:20868728 | pubmed:meshHeading | pubmed-meshheading:20868728... | lld:pubmed |
| pubmed-article:20868728 | pubmed:meshHeading | pubmed-meshheading:20868728... | lld:pubmed |
| pubmed-article:20868728 | pubmed:meshHeading | pubmed-meshheading:20868728... | lld:pubmed |
| pubmed-article:20868728 | pubmed:meshHeading | pubmed-meshheading:20868728... | lld:pubmed |
| pubmed-article:20868728 | pubmed:meshHeading | pubmed-meshheading:20868728... | lld:pubmed |
| pubmed-article:20868728 | pubmed:meshHeading | pubmed-meshheading:20868728... | lld:pubmed |
| pubmed-article:20868728 | pubmed:meshHeading | pubmed-meshheading:20868728... | lld:pubmed |
| pubmed-article:20868728 | pubmed:meshHeading | pubmed-meshheading:20868728... | lld:pubmed |
| pubmed-article:20868728 | pubmed:meshHeading | pubmed-meshheading:20868728... | lld:pubmed |
| pubmed-article:20868728 | pubmed:meshHeading | pubmed-meshheading:20868728... | lld:pubmed |
| pubmed-article:20868728 | pubmed:meshHeading | pubmed-meshheading:20868728... | lld:pubmed |
| pubmed-article:20868728 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20868728 | pubmed:articleTitle | U373-MG cells express PepT2 and accumulate the fluorescently tagged dipeptide-derivative ?-Ala-Lys-N(?)-AMCA. | lld:pubmed |
| pubmed-article:20868728 | pubmed:affiliation | Zentralinstitut für Laboratoriumsmedizin und Pathobiochemie, Charité-Universitätsmedizin Berlin, Campus Virchow Klinikum, Augustenburger Platz 1, 13353 Berlin, Germany. mathias.zimmermann@charite.de | lld:pubmed |
| pubmed-article:20868728 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:6565 | entrezgene:pubmed | pubmed-article:20868728 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20868728 | lld:entrezgene |
