Linked Life Data

2086578

Source:http://linkedlifedata.com/resource/pubmed/id/2086578

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1991-5-20
pubmed:abstractText
Nuclear androgen receptor dynamics were analyzed in peritubular cells and compared with those in cultured Sertoli cells. Nuclear receptors with a high affinity for [3H]dimethylnortestosterone (DMNT; mibolerone) exhibited equilibrium constants of 0.8 and 0.7 nmol, in Sertoli and peritubular cells, respectively. Time- and dose-dependent accumulation of nuclear bound receptors after exposure of whole cells to [3H]testosterone was similar for both cell types. Exogenously administered ligands demonstrated similar relative potencies as competitors with [3H]T for Sertoli and peritubular cell nuclear binding sites: DMNT greater than T greater than medroxyprogesterone acetate (MPA) greater than cyproterone acetate (CPA) tau hydroxyflutamide (OHF). Cells incubated with T or MPA showed increased nuclear androgen receptor concentrations compared to untreated controls, whereas those treated with CPA or OHF did not. These results demonstrate that the nuclear androgen receptor dynamics of peritubular cells are consistent with those of target cells. Since the dynamics are similar in Sertoli and peritubular cells, both cell types have the potential to respond to local androgen concentrations and may play important roles in androgen-dependent effects on seminiferous tubule function.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0196-3635
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
514-20
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:articleTitle
Nuclear androgen receptor dynamics in testicular peritubular and Sertoli cells.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77030.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.