Linked Life Data

20865327

Source:http://linkedlifedata.com/resource/pubmed/id/20865327

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2011-4-7
pubmed:abstractText
Agouti-related peptide (AgRP), a brain neuropeptide generated by AgRP/neuropeptide Y (NPY) neurons, plays a vital role in the hypothalamic regulation of energy homeostasis. RT-PCR and real-time PCR were carried out in various tissues to detect the AgRP expression pattern in pigs. Our RT-PCR results showed that the pig AgRP gene was ubiquitously expressed in all examined tissues including heart, liver, spleen, lung, kidney, stomach, bladder, m. longissimus, belly fat, brain, large intestine, lymph, back fat, skin, and hypothalamus. Real-time quantitative PCR experiments revealed that it is in the hypothalamus with the highest expression of AgRP both in adult Lantang and Landrace pigs compared to the back fat and m.longissimus muscle and the cDNA level of AgRP in the hypothalamus of adult Chinese indigenous Lantang pig (fat-type) is significantly higher than that of Landrace pig (lean-type). To understand the regulation of the pig AgRP gene, the 5'-flanking region was isolated from a pig bacterial artificial chromosome library and used in a luciferase reporter assay. A positive cis-acting element for efficient AgRP expression was identified at nucleotides -501 to -479, by 5'-serial deletion of the promoter. Electrophoretic mobility-shift assays (EMSA) with competing oligonucleotides revealed that the critical region contained a cis-acting element for Neurogenic Differentiation (NeuroD), which is a member of the NeuroD family of basic-helix-loop-helix transcription factors. This element has not been reported in human or mouse AgRP genes. Our results indicated that NeuroD might be an essential regulatory factor for transcription of pig AgRP, providing an important clue about energy homeostasis regulation in the porcine and human brain.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1573-4978
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2233-9
pubmed:meshHeading
pubmed-meshheading:20865327-5' Flanking Region, pubmed-meshheading:20865327-Agouti-Related Protein, pubmed-meshheading:20865327-Animals, pubmed-meshheading:20865327-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:20865327-Chromosomes, Artificial, Bacterial, pubmed-meshheading:20865327-Cloning, Molecular, pubmed-meshheading:20865327-DNA Primers, pubmed-meshheading:20865327-Electrophoretic Mobility Shift Assay, pubmed-meshheading:20865327-Energy Metabolism, pubmed-meshheading:20865327-Gene Expression Profiling, pubmed-meshheading:20865327-Luciferases, pubmed-meshheading:20865327-Nerve Tissue Proteins, pubmed-meshheading:20865327-Oligonucleotides, pubmed-meshheading:20865327-Polymerase Chain Reaction, pubmed-meshheading:20865327-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:20865327-Species Specificity, pubmed-meshheading:20865327-Swine
pubmed:year
2011
pubmed:articleTitle
Cloning and characterization of the 5'-flanking region of the pig AgRP gene.
pubmed:affiliation
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't