Linked Life Data

20863889

Source:http://linkedlifedata.com/resource/pubmed/id/20863889

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2010-12-7
pubmed:abstractText
Large plasmids, presumably replicating via the theta mechanism, have been identified in numerous gram-positive bacteria. However, their characterization is rather poor and predominantly limited to those harbored by some (opportunistic) pathogenic bacteria. Here we determined the DNA sequence of the 43.3 kb plasmid p576 from Bacillus pumilus strain NRS576, the first B. pumilus theta-replicating plasmid sequenced. Plasmid p576 has a modular structure, but surprisingly, it does not seem to encode a Rep protein found on most theta-replicating plasmids. However, a ?1 kb region was identified showing homology with the Rep-independent replication region of Bacillus subtilis plasmid pLS20, and we demonstrated that this region is sufficient for autonomous replication. The plasmid contains various large direct repeat sequences. A likely function could be attributed to at least 15 putative p576 genes. Some of these are predicted to be involved in stable maintenance of the plasmid; others are likely to encode proteins involved in conjugation. p576 also carries a rap-phr cassette whose possible function is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1769-7123
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Institut Pasteur. Published by Elsevier SAS. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
161
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
772-82
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Complete nucleotide sequence and determination of the replication region of the sporulation inhibiting plasmid p576 from Bacillus pumilus NRS576.
pubmed:affiliation
Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), Instituto de Biología Molecular "Eladio Viñuela" (CSIC), Universidad Autónoma, Canto Blanco, 28049 Madrid, Spain. psingh@cbm.uam.es
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't