Linked Life Data

20863808

Source:http://linkedlifedata.com/resource/pubmed/id/20863808

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-11-12
pubmed:abstractText
Here we describe a technique we have named polymerase chain reaction stem-loop conformational polymorphism (PCR-SLCP) analysis for screening DNA sequence variation. This technique uses PCR primers with adapters at the 5' ends to generate amplicons containing inverted terminal repeat sequences. These enable the formation of specific conformers for single-stranded molecules on denaturation, and sequence variation in the loop region may affect the structure of these and their mobilities during electrophoresis. The technique has the ability to resolve sequence variation in amplicons that cannot be resolved using PCR single-strand conformational polymorphism (PCR-SSCP). PCR-SLCP is simple and sensitive, and the results are highly repeatable.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1096-0309
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
408
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
340-1
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Detection of sequence variation and genotyping of polymorphic genes using polymerase chain reaction stem-loop conformational polymorphism analysis.
pubmed:affiliation
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou, Gansu 730070, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't