Source:http://linkedlifedata.com/resource/pubmed/id/20859951
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
20
|
| pubmed:dateCreated |
2010-10-15
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| pubmed:abstractText |
Novel tools are necessary to explore proteins related to human immunodeficiency virus (HIV) infection. In this work, proteomic and glycoproteomic technology were employed to examine plasma samples from HIV-positive patients. Through comparative proteome analysis of normal and HIV-positive plasma samples, 19 differentially expressed protein spots related to 12 non-redundant proteins were identified by ESI-ion trap MS. Among these, the 130-kDa isoform of ?-1-antitrypsin was found to be decreased in HIV-positive patients while another variant with a molecular weight of 40 kDa was increased. SWISS-2-D-PAGE reference gel and protein sequence comparisons of the 40-kDa protein showed homology with ?-1-antitrypsin minus the N-terminus, and its identity was further confirmed by 1-D Western blotting and glycoproteomic analysis. In all, our results showed that proteomics and glycoproteomics are powerful tools for discovering proteins related to HIV infection. Furthermore, this 40-kDa variant of ?-1-antitrypsin found in the plasma of HIV-positive individuals may prove to be a potentially useful biomarker for anti-HIV research according to bioinformatics analysis.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
1522-2683
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| pubmed:author |
pubmed-author:ChaoQiuQ,
pubmed-author:JiaXiaofangX,
pubmed-author:JosKK,
pubmed-author:KIDDG PGP,
pubmed-author:LiuLiwenL,
pubmed-author:LuHongzhouH,
pubmed-author:MaFangF,
pubmed-author:ShenFangF,
pubmed-author:ShenYinzhongY,
pubmed-author:SunJianjunJ,
pubmed-author:WooM KMK,
pubmed-author:YangPengyuanP,
pubmed-author:YaoYaminY,
pubmed-author:ZhangLijunL,
pubmed-author:ZhangXiaojunX
|
| pubmed:issnType |
Electronic
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| pubmed:volume |
31
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3437-45
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| pubmed:meshHeading |
pubmed-meshheading:20859951-Adult,
pubmed-meshheading:20859951-Amino Acid Sequence,
pubmed-meshheading:20859951-Biological Markers,
pubmed-meshheading:20859951-Blotting, Western,
pubmed-meshheading:20859951-Case-Control Studies,
pubmed-meshheading:20859951-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:20859951-Female,
pubmed-meshheading:20859951-Glycoproteins,
pubmed-meshheading:20859951-Glycosylation,
pubmed-meshheading:20859951-HIV Infections,
pubmed-meshheading:20859951-Humans,
pubmed-meshheading:20859951-Male,
pubmed-meshheading:20859951-Middle Aged,
pubmed-meshheading:20859951-Molecular Sequence Data,
pubmed-meshheading:20859951-Molecular Weight,
pubmed-meshheading:20859951-Protein Isoforms,
pubmed-meshheading:20859951-Proteomics,
pubmed-meshheading:20859951-Reproducibility of Results,
pubmed-meshheading:20859951-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:20859951-alpha 1-Antitrypsin
|
| pubmed:year |
2010
|
| pubmed:articleTitle |
Alpha-1 antitrypsin variants in plasma from HIV-infected patients revealed by proteomic and glycoproteomic analysis.
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| pubmed:affiliation |
Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, PR China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|