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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2010-11-8
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pubmed:abstractText |
We carried out a model of chronic inflammation using a subcutaneous paraffin tablet in mice experimentally infected with Leishmania major. It was previously reported that the parasite load following paraffin implantation occurred at a peak of 21 days in both BALB/c and C57BL/6 mice. At the present study, we have investigated what cytokines and chemokines are directly related to the parasite load in C57BL/6 mice. All mice were divided in four groups: mice implanted with paraffin tablets; mice experimentally infected with L. major; mice implanted with paraffin tablets and experimentally infected with L. major; and mice submitted only to the surgery were used for the Real-Time Polymerase Chain Reaction (RT-PCR) controls. Fragments of skin tissue and the tissue surrounding the paraffin tablets (inflammatory capsule) were collected for histopathology and RT-PCR studies. By 21 days, a diffuse chronic inflammatory reaction was mainly observed in the deep dermis where macrophages parasitized with Leishmania amastigotes were also found. RT-PCR analysis has shown that BALB/c mice showed strong IL-4 and IL-10 mRNA expression than controls with very little expression of IFN-?. In contrast, both IFN-? and IL-10 mRNA was found in higher levels in C57BL/6 animals. Moreover, in C57BL/6 mice the expression of chemokines mRNA of CCL3/MIP-1? was more highly expressed than CCL2/MCP-1. We conclude that the Th1 immune response C57BL/6 did not change to a Th2 response, even though C57BL/6 animals presented higher parasitism than BALB/c mice 21 days after infection and paraffin implantation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20857143-10338536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20857143-10807766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20857143-10859337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20857143-10881180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20857143-10897418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20857143-10899907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20857143-11093125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20857143-11097369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20857143-11113254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20857143-11145695,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20857143-12102714,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20857143-15271961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20857143-15275451,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20857143-9504340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20857143-9782133
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1432-2307
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
457
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
609-18
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:20857143-Animals,
pubmed-meshheading:20857143-Chemokines,
pubmed-meshheading:20857143-Cytokines,
pubmed-meshheading:20857143-Leishmania major,
pubmed-meshheading:20857143-Leishmaniasis, Cutaneous,
pubmed-meshheading:20857143-Mice,
pubmed-meshheading:20857143-Mice, Inbred BALB C,
pubmed-meshheading:20857143-Mice, Inbred C57BL,
pubmed-meshheading:20857143-Paraffin,
pubmed-meshheading:20857143-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:20857143-Tablets
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pubmed:year |
2010
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pubmed:articleTitle |
Murine immune response induced by Leishmania major during the implantation of paraffin tablets.
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pubmed:affiliation |
Departamento de Patologia Geral, Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Belo Horizonte, MG, Brazil. marialeticia@ufmg.br
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pubmed:publicationType |
Journal Article
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