| pubmed-article:20854851 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20854851 | lifeskim:mentions | umls-concept:C0032846 | lld:lifeskim |
| pubmed-article:20854851 | lifeskim:mentions | umls-concept:C0007585 | lld:lifeskim |
| pubmed-article:20854851 | lifeskim:mentions | umls-concept:C0037494 | lld:lifeskim |
| pubmed-article:20854851 | lifeskim:mentions | umls-concept:C1704410 | lld:lifeskim |
| pubmed-article:20854851 | lifeskim:mentions | umls-concept:C0205217 | lld:lifeskim |
| pubmed-article:20854851 | lifeskim:mentions | umls-concept:C0311417 | lld:lifeskim |
| pubmed-article:20854851 | lifeskim:mentions | umls-concept:C0181586 | lld:lifeskim |
| pubmed-article:20854851 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:20854851 | pubmed:dateCreated | 2010-11-15 | lld:pubmed |
| pubmed-article:20854851 | pubmed:abstractText | The PR-10a protein (formerly STH-2) is known to be induced by biotic stress in potato. The present study demonstrates that transgenic suspension cells of the potato cultivar Desiree over-expressing the PR-10a protein exhibit significantly increased salt and osmotic tolerance compared to the respective wild type cells. A comparison of the proteome pattern of Solanum tuberosum suspension cultures cv. Desiree before and after the treatment with NaCl or sorbitol under equiosmolar conditions (740mOs/kg) revealed the pathogenesis related protein PR-10a to be one of the predominant differentially expressed proteins in potato cell cultures. The pr-10a mRNA was confirmed to be present by RT-PCR from salt challenged suspension cells and was transcribed into cDNA. For PR-10a over-expression Agrobacterium tumefaciens mediated transformation of the potato cells and a dicistronic vector harboring the cDNA of the pr-10a gene linked to a luciferase gene by an IRES (Internal Ribosome Binding Site) was used. The IRES mediated translation leads to co-expression of PR-10a and luciferase in a fixed ratio. By non-invasive luciferase assay homologous PR-10a over-expressing callus was identified after selection on phosphinothricin supplemented medium. This callus was used for the setup of a transgenic suspension culture. Along with increased salt and osmotic tolerance the transformed culture showed changed proline and glutathione levels under abiotic stress conditions in comparison to the wild type. | lld:pubmed |
| pubmed-article:20854851 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20854851 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20854851 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20854851 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20854851 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20854851 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20854851 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20854851 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20854851 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20854851 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20854851 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20854851 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20854851 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20854851 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20854851 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:20854851 | pubmed:issn | 1873-4863 | lld:pubmed |
| pubmed-article:20854851 | pubmed:author | pubmed-author:ZabikR MRM | lld:pubmed |
| pubmed-article:20854851 | pubmed:author | pubmed-author:WissingJoseph... | lld:pubmed |
| pubmed-article:20854851 | pubmed:author | pubmed-author:HajirezaeiMoh... | lld:pubmed |
| pubmed-article:20854851 | pubmed:author | pubmed-author:JacobsenHans-... | lld:pubmed |
| pubmed-article:20854851 | pubmed:author | pubmed-author:KieseckerHeik... | lld:pubmed |
| pubmed-article:20854851 | pubmed:author | pubmed-author:SchumacherHei... | lld:pubmed |
| pubmed-article:20854851 | pubmed:author | pubmed-author:Heine-Dobbern... | lld:pubmed |
| pubmed-article:20854851 | pubmed:author | pubmed-author:El-BannaAntar... | lld:pubmed |
| pubmed-article:20854851 | pubmed:author | pubmed-author:VaasLeaL | lld:pubmed |
| pubmed-article:20854851 | pubmed:copyrightInfo | Copyright © 2010 Elsevier B.V. All rights reserved. | lld:pubmed |
| pubmed-article:20854851 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20854851 | pubmed:volume | 150 | lld:pubmed |
| pubmed-article:20854851 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20854851 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20854851 | pubmed:pagination | 277-87 | lld:pubmed |
| pubmed-article:20854851 | pubmed:meshHeading | pubmed-meshheading:20854851... | lld:pubmed |
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| pubmed-article:20854851 | pubmed:meshHeading | pubmed-meshheading:20854851... | lld:pubmed |
| pubmed-article:20854851 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20854851 | pubmed:articleTitle | Over-expression of PR-10a leads to increased salt and osmotic tolerance in potato cell cultures. | lld:pubmed |
| pubmed-article:20854851 | pubmed:affiliation | German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstr. 7b, D-38124 Braunschweig, Germany. | lld:pubmed |
| pubmed-article:20854851 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20854851 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
