Linked Life Data

20854851

Source:http://linkedlifedata.com/resource/pubmed/id/20854851

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-11-15
pubmed:abstractText
The PR-10a protein (formerly STH-2) is known to be induced by biotic stress in potato. The present study demonstrates that transgenic suspension cells of the potato cultivar Desiree over-expressing the PR-10a protein exhibit significantly increased salt and osmotic tolerance compared to the respective wild type cells. A comparison of the proteome pattern of Solanum tuberosum suspension cultures cv. Desiree before and after the treatment with NaCl or sorbitol under equiosmolar conditions (740mOs/kg) revealed the pathogenesis related protein PR-10a to be one of the predominant differentially expressed proteins in potato cell cultures. The pr-10a mRNA was confirmed to be present by RT-PCR from salt challenged suspension cells and was transcribed into cDNA. For PR-10a over-expression Agrobacterium tumefaciens mediated transformation of the potato cells and a dicistronic vector harboring the cDNA of the pr-10a gene linked to a luciferase gene by an IRES (Internal Ribosome Binding Site) was used. The IRES mediated translation leads to co-expression of PR-10a and luciferase in a fixed ratio. By non-invasive luciferase assay homologous PR-10a over-expressing callus was identified after selection on phosphinothricin supplemented medium. This callus was used for the setup of a transgenic suspension culture. Along with increased salt and osmotic tolerance the transformed culture showed changed proline and glutathione levels under abiotic stress conditions in comparison to the wild type.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1873-4863
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
150
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
277-87
pubmed:meshHeading
pubmed-meshheading:20854851-Blotting, Southern, pubmed-meshheading:20854851-Cells, Cultured, pubmed-meshheading:20854851-DNA-Binding Proteins, pubmed-meshheading:20854851-Electrophoresis, Gel, Two-Dimensional, pubmed-meshheading:20854851-Glutathione, pubmed-meshheading:20854851-Nuclear Proteins, pubmed-meshheading:20854851-Osmotic Pressure, pubmed-meshheading:20854851-Plant Proteins, pubmed-meshheading:20854851-Proline, pubmed-meshheading:20854851-RNA, Messenger, pubmed-meshheading:20854851-RNA, Plant, pubmed-meshheading:20854851-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:20854851-Sodium Chloride, pubmed-meshheading:20854851-Solanum tuberosum, pubmed-meshheading:20854851-Sorbitol, pubmed-meshheading:20854851-Stress, Physiological, pubmed-meshheading:20854851-Transformation, Genetic
pubmed:year
2010
pubmed:articleTitle
Over-expression of PR-10a leads to increased salt and osmotic tolerance in potato cell cultures.
pubmed:affiliation
German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstr. 7b, D-38124 Braunschweig, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't