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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2010-9-20
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pubmed:abstractText |
Understanding the functional complexity of protein interactions requires mapping biomolecular complexes within the cellular environment over biologically relevant time scales. Herein, we describe a set of reversible multicolored heteroprotein complementation fragments based on various firefly and click beetle luciferases that utilize the same substrate, D-luciferin. Luciferase heteroprotein fragment complementation systems enabled dual-color quantification of two discrete pairs of interacting proteins simultaneously or two distinct proteins interacting with a third shared protein in live cells. Using real-time analysis of click beetle green and click beetle red luciferase heteroprotein fragment complementation applied to ?-TrCP, an E3-ligase common to the regulation of both ?-catenin and I?B?, GSK3? was identified as a candidate kinase regulating I?B? processing. These dual-color protein interaction switches may enable directed dynamic analysis of a variety of protein interactions in living cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1879-1301
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2010 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
24
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1018-29
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pubmed:dateRevised |
2011-11-2
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pubmed:meshHeading |
pubmed-meshheading:20851351-Animals,
pubmed-meshheading:20851351-Beetles,
pubmed-meshheading:20851351-Benzothiazoles,
pubmed-meshheading:20851351-Cell Line,
pubmed-meshheading:20851351-Color,
pubmed-meshheading:20851351-Glycogen Synthase Kinase 3,
pubmed-meshheading:20851351-Humans,
pubmed-meshheading:20851351-Immunosuppressive Agents,
pubmed-meshheading:20851351-Luciferases,
pubmed-meshheading:20851351-Protein Interaction Mapping,
pubmed-meshheading:20851351-Sirolimus,
pubmed-meshheading:20851351-beta Catenin,
pubmed-meshheading:20851351-beta-Transducin Repeat-Containing Proteins
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pubmed:year |
2010
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pubmed:articleTitle |
Dual-color click beetle luciferase heteroprotein fragment complementation assays.
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pubmed:affiliation |
Molecular Imaging Center, Mallinckrodt Institute of Radiology, Department of Developmental Biology, Washington University, St. Louis, MO 63110, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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