Linked Life Data

20850859

Source:http://linkedlifedata.com/resource/pubmed/id/20850859

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-10-11
pubmed:abstractText
Vpu enhances HIV-1 release by suppressing CD317-mediated tethering of virions to the cell surface. In HeLa H1 cells, Vpu(+) infection produces significant amounts of tethered virions, although efficient virus release requires Vpu. We have analyzed membrane targeting and assembly of newly synthesized Gag in infected HeLa H1 cells by quantitative pulse-chase assays in both Vpu(+) and ?Vpu virus backgrounds and in the presence and absence of CD317. Our results show that formation of tethered virions precedes release of viruses to the culture medium in the Vpu(+) infection, and CD317 knockdown reduces tethering in both Vpu(+) and ?Vpu virus backgrounds. Significantly, our results indicate that tethered Vpu(+) viruses represent precursors for extracellular viruses, and Vpu thus appears to reverse tethering in HeLa H1 cells after budding.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1096-0341
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
25
pubmed:volume
407
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
289-95
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Tethered virions are intermediates in the assembly and release of HIV-1 particles.
pubmed:affiliation
Department of Virology, Haartman Institute, PO Box 21, FIN-00014 University of Helsinki, Finland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't