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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2010-9-20
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pubmed:abstractText |
Gamma protocadherins (Pcdh-?s) resemble classical cadherins and have the potential to engage in cell-cell interactions with homophilic properties. Emerging evidence suggests non-conventional roles for some protocadherins in neural development. We sought to determine whether Pcdh-? trafficking in neurons is consistent with an intracellular role for these molecules. Here we show that, in contrast to the largely surface localization of classical cadherins, endogenous Pcdh-?s are primarily intracellular in rat neurons in vivo and are equally distributed within organelles of subsynaptic dendritic and axonal compartments. A strikingly higher proportion of Pcdh-?-containing organelles in synaptic compartments was observed at postnatal day 16. To determine the origin of Pcdh-?-trafficking organelles, we isolated organelles with Pcdh-? antibody-coupled magnetic beads from brain organelle suspensions. Vesicles with high levels of COPII and endoplasmic reticulum-Golgi intermediate compartment (ERGIC) components were isolated with the Pcdh-? antibody but not with the classical cadherin antibody. In cultured hippocampal neurons, Pcdh-? immunolabeling partially overlapped with calnexin- and COPII-positive puncta in dendrites. Mobile Pcdh-?-GFP profiles dynamically codistributed with a DsRed construct coupled to ER retention signals by live imaging. Pcdh-? expression correlated with accumulations of tubulovesicular and ER-like organelles in dendrites. Our results are consistent with the possibility that Pcdh-?s could have a unique function within the secretory pathway in addition to their documented surface roles.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20849527-10202138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20849527-10337493,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20849527-8909549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20849527-9655502
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1460-9568
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pubmed:author |
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pubmed:copyrightInfo |
© 2010 The Authors. European Journal of Neuroscience © 2010 Federation of European Neuroscience Societies and Blackwell Publishing Ltd.
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pubmed:issnType |
Electronic
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
921-31
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pubmed:dateRevised |
2011-9-13
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pubmed:meshHeading |
pubmed-meshheading:20849527-Animals,
pubmed-meshheading:20849527-Cadherins,
pubmed-meshheading:20849527-Cells, Cultured,
pubmed-meshheading:20849527-Neurons,
pubmed-meshheading:20849527-Protein Transport,
pubmed-meshheading:20849527-Rats,
pubmed-meshheading:20849527-Rats, Sprague-Dawley,
pubmed-meshheading:20849527-Secretory Pathway,
pubmed-meshheading:20849527-Secretory Vesicles
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pubmed:year |
2010
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pubmed:articleTitle |
?-protocadherins are enriched and transported in specialized vesicles associated with the secretory pathway in neurons.
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pubmed:affiliation |
Department of Neuroscience, Mount Sinai School of Medicine, New York, NY, USA.
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