Source:http://linkedlifedata.com/resource/pubmed/id/20839920
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2010-9-29
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pubmed:abstractText |
Human immunodeficiency virus type 1 (HIV-1) and viral proteins affect neuronal survival and neuron-glial cell interactions, which culminate in neurological disorders. HIV-1 infects regions of neurogenesis in human adult and pediatric brain. However, little is known about the effect of HIV-1 or viral proteins on the properties of human neural precursor cells (hNPCs), particularly neurogenesis, hence a detailed investigation on these lines is warranted. Human neural precursor cells were cultured in presence and absence of HIV-1B transactivating protein Tat to investigate if HIV-1 viral protein alters the properties of human neural precursor cells. Cellular and molecular approaches were adopted to study the effect of HIV-1B transactivating protein Tat on proliferation and differentiation potential of human fetal brain-derived NPCs. Cell proliferation assays such as BrdU and Ki67 staining and pathway-specific cDNA and protein arrays were used in the study. Data reveal that HIV-1B Tat protein severely affects proliferation of hNPCs, as evident by lower incorporation of BrdU and Ki67 staining as well as neurosphere assay. HIV-1 Tat substantially attenuated neurogenesis, as evident by the smaller numbers of Tuj-1- and doublecortin-positive cells differentiated from hNPCs, without affecting their viability. These data suggest that HIV-1 Tat alters the properties of human neural precursor cells via attenuation of the cell cycle regulatory unit cyclin D1 and the mitogen-activated protein kinase (MAPK) pathway, particularly extracellular signal-related kinase 1/2 (ERK1/2). The study provides new insights into cellular and molecular mechanisms that may modulate human neural precursor cell properties in HIV/AIDS (acquired immunodeficiency syndrome) individuals. Validation with autopsy brain samples is necessary to further substantiate these important observations.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1538-2443
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
355-67
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pubmed:meshHeading |
pubmed-meshheading:20839920-AIDS Dementia Complex,
pubmed-meshheading:20839920-Brain,
pubmed-meshheading:20839920-Cells, Cultured,
pubmed-meshheading:20839920-Cyclin D1,
pubmed-meshheading:20839920-Down-Regulation,
pubmed-meshheading:20839920-HIV-1,
pubmed-meshheading:20839920-Humans,
pubmed-meshheading:20839920-Neural Stem Cells,
pubmed-meshheading:20839920-Neurogenesis,
pubmed-meshheading:20839920-Recombinant Proteins,
pubmed-meshheading:20839920-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2010
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pubmed:articleTitle |
Human immunodeficiency virus type 1 Tat modulates proliferation and differentiation of human neural precursor cells: implication in NeuroAIDS.
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pubmed:affiliation |
Molecular and Cellular Neuroscience, National Brain Research Center, Manesar, Haryana, India.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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