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rdf:type |
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lifeskim:mentions |
umls-concept:C0013030,
umls-concept:C0030567,
umls-concept:C0033684,
umls-concept:C0086418,
umls-concept:C0087111,
umls-concept:C0392747,
umls-concept:C0439849,
umls-concept:C0443172,
umls-concept:C0445223,
umls-concept:C0449774,
umls-concept:C0678632,
umls-concept:C0815000,
umls-concept:C1424222,
umls-concept:C1552599,
umls-concept:C1704787
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pubmed:issue |
9
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pubmed:dateCreated |
2010-9-30
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pubmed:abstractText |
DJ-1 is a mitochondrial protein linked to Parkinson's disease. DJ-1 has been suggested to have several possible functions, although it has been mainly associated to oxidative stress defence. Changes in the two-dimensional electrophoresis pattern have been thoroughly described as a consequence of oxidative modification of the Cys106 residue. There is accumulating evidence supporting a specific role of DJ-1 in protecting dopaminergic neurons from dopamine itself. By exposing SH-SY5Y human neuroblastoma catecholaminergic cells to dopamine, we observed a specific increase in the most acidic forms in the DJ-1 two-dimensional electrophoresis pattern together with a significant decrease of the most basic spot. Unlike cells exposed to generic oxidative conditions, no additional shift was observed. The results are corroborated by a meta-analysis of the literature showing that in the absence of dopamine treatment the specific acidic form is underrepresented.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1521-6551
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pubmed:author |
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pubmed:copyrightInfo |
© 2010 IUBMB IUBMB Life.
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pubmed:issnType |
Electronic
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
688-92
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pubmed:meshHeading |
pubmed-meshheading:20836127-Blotting, Western,
pubmed-meshheading:20836127-Cell Line, Tumor,
pubmed-meshheading:20836127-Dopamine,
pubmed-meshheading:20836127-Dopamine Agents,
pubmed-meshheading:20836127-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:20836127-Flow Cytometry,
pubmed-meshheading:20836127-Humans,
pubmed-meshheading:20836127-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:20836127-Neuroblastoma,
pubmed-meshheading:20836127-Oncogene Proteins,
pubmed-meshheading:20836127-Parkinson Disease,
pubmed-meshheading:20836127-Protein Isoforms
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pubmed:year |
2010
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pubmed:articleTitle |
Changes in the two-dimensional electrophoresis pattern of the Parkinson's disease related protein DJ-1 in human SH-SY5Y neuroblastoma cells after dopamine treatment.
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pubmed:affiliation |
Department of Structural and Functional Biology, Center of Neuroscience, University of Insubria, Busto Arsizio, VA, Italy.
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pubmed:publicationType |
Journal Article
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