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rdf:type |
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lifeskim:mentions |
umls-concept:C0033684,
umls-concept:C0041904,
umls-concept:C0052418,
umls-concept:C0127400,
umls-concept:C0162493,
umls-concept:C0184512,
umls-concept:C0205263,
umls-concept:C0215848,
umls-concept:C0243043,
umls-concept:C1171362,
umls-concept:C1418576,
umls-concept:C1418577,
umls-concept:C1423080,
umls-concept:C1423613,
umls-concept:C1822686,
umls-concept:C2348110,
umls-concept:C2348977
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pubmed:issue |
3
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pubmed:dateCreated |
2011-1-14
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pubmed:abstractText |
Hypoxia-inducible factor-1? (HIF-1?) has been reported to regulate over 100 gene expressions in response to hypoxia and other stress conditions. In the present study, we found that arsenite could induce HIF-1? protein accumulation in both mouse epidermal Cl41 cells and mouse embryonic fibroblasts (MEFs). Knockout of p85?, a regulatory subunit of PI-3K, in MEFs (p85?(-/-)) dramatically decreased the arsenite-induced HIF-1? accumulation, indicating that p85? is crucial for arsenite effects on the stabilization of HIF-1? protein. Our further studies suggest that arsenite could induce inducible Hsp70 expression, and transfection of inducible Hsp70 into p85?(-/-) MEFs could restore HIF-1? protein accumulation. Moreover, the results using EMSA and Supershift assays indicate that p85? is crucial for arsenite-induced activation of the heat-shock transcription factor 1 (HSF-1), which is responsible for transcription of inducible Hsp70. Taken together, p85?-mediated HIF-1? stabilization upon arsenite exposure is specifically through HSF-1 activation and subsequent up-regulation of the inducible Hsp70 expression.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arsenites,
http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens,
http://linkedlifedata.com/resource/pubmed/chemical/Class Ia Phosphatidylinositol...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HSP70 Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HSP90 Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hsf1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Hypoxia-Inducible Factor 1, alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt,
http://linkedlifedata.com/resource/pubmed/chemical/Teratogens,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/arsenite
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1420-9071
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pubmed:author |
pubmed-author:GuoWeiW,
pubmed-author:HuangChuanshuC,
pubmed-author:LiJingxiaJ,
pubmed-author:LiXueyongX,
pubmed-author:LiuJinyiJ,
pubmed-author:ShiXianglinX,
pubmed-author:XiaQingQ,
pubmed-author:YangZhuoZ,
pubmed-author:YuYonghuiY,
pubmed-author:ZhangDongyunD,
pubmed-author:ZuoZhenghongZ
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pubmed:issnType |
Electronic
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
475-88
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pubmed:meshHeading |
pubmed-meshheading:20835880-Animals,
pubmed-meshheading:20835880-Arsenites,
pubmed-meshheading:20835880-Carcinogens,
pubmed-meshheading:20835880-Cell Line,
pubmed-meshheading:20835880-Cell Nucleolus,
pubmed-meshheading:20835880-Class Ia Phosphatidylinositol 3-Kinase,
pubmed-meshheading:20835880-DNA-Binding Proteins,
pubmed-meshheading:20835880-Epidermis,
pubmed-meshheading:20835880-Fibroblasts,
pubmed-meshheading:20835880-Gene Expression Regulation,
pubmed-meshheading:20835880-HSP70 Heat-Shock Proteins,
pubmed-meshheading:20835880-HSP90 Heat-Shock Proteins,
pubmed-meshheading:20835880-Hypoxia-Inducible Factor 1, alpha Subunit,
pubmed-meshheading:20835880-Mice,
pubmed-meshheading:20835880-Protein Stability,
pubmed-meshheading:20835880-Protein Transport,
pubmed-meshheading:20835880-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:20835880-Teratogens,
pubmed-meshheading:20835880-Transcription Factors,
pubmed-meshheading:20835880-Up-Regulation
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pubmed:year |
2011
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pubmed:articleTitle |
Arsenite stabilizes HIF-1? protein through p85?-mediated up-regulation of inducible Hsp70 protein expression.
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pubmed:affiliation |
Nelson Institute of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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