| pubmed-article:20835816 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20835816 | lifeskim:mentions | umls-concept:C0018026 | lld:lifeskim |
| pubmed-article:20835816 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:20835816 | lifeskim:mentions | umls-concept:C0600364 | lld:lifeskim |
| pubmed-article:20835816 | lifeskim:mentions | umls-concept:C1705425 | lld:lifeskim |
| pubmed-article:20835816 | lifeskim:mentions | umls-concept:C0181496 | lld:lifeskim |
| pubmed-article:20835816 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:20835816 | lifeskim:mentions | umls-concept:C0013812 | lld:lifeskim |
| pubmed-article:20835816 | lifeskim:mentions | umls-concept:C0069299 | lld:lifeskim |
| pubmed-article:20835816 | lifeskim:mentions | umls-concept:C1522485 | lld:lifeskim |
| pubmed-article:20835816 | lifeskim:mentions | umls-concept:C0599013 | lld:lifeskim |
| pubmed-article:20835816 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:20835816 | pubmed:dateCreated | 2010-10-20 | lld:pubmed |
| pubmed-article:20835816 | pubmed:abstractText | A highly selective electrochemiluminescent biosensor for the detection of target nephrotoxic toxin, ochratoxin A (OTA), was developed using a DNA aptamer as the recognition element and N-(4-aminobutyl)-N-ethylisoluminol (ABEI) as the signal-producing compound. The electrochemiluminescent aptamer biosensor was fabricated by immobilizing aptamer complementary DNA 1 sequence onto the surface of a gold-nanoparticle (AuNP)-modified gold electrode. ABEI-labeled aptamer DNA 2 sequence hybridized to DNA 1 and was utilized as an electrochemiluminescent probe. A decreased electrochemiluminescence (ECL) signal was generated upon aptamer recognition of the target OTA, which induced the dissociation of DNA 2 (ABEI-labeled aptamer electrochemiluminescent probe) from DNA 1 and moved it far away from the electrode surface. Under the optimal conditions, the decreased ECL intensity was proportional to an OTA concentration ranging from 0.02 to 3.0 ng mL(-1), with a detection limit of 0.007 ng mL(-1). The relative standard deviation was 3.8% at 0.2 ng mL(-1) (n = 7). The proposed method has been applied to measure OTA in naturally contaminated wheat samples and validated by an official method. This work demonstrates the combination of a highly binding aptamer with a highly sensitive ECL technique to design an electrochemiluminescent biosensor, which is a very promising approach for the determination of small-molecule toxins. | lld:pubmed |
| pubmed-article:20835816 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20835816 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20835816 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20835816 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20835816 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20835816 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20835816 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20835816 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20835816 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20835816 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20835816 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:20835816 | pubmed:issn | 1618-2650 | lld:pubmed |
| pubmed-article:20835816 | pubmed:author | pubmed-author:QiK YKY | lld:pubmed |
| pubmed-article:20835816 | pubmed:author | pubmed-author:WangZhoupingZ | lld:pubmed |
| pubmed-article:20835816 | pubmed:author | pubmed-author:DuanNuoN | lld:pubmed |
| pubmed-article:20835816 | pubmed:author | pubmed-author:WuShijiaS | lld:pubmed |
| pubmed-article:20835816 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20835816 | pubmed:volume | 398 | lld:pubmed |
| pubmed-article:20835816 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20835816 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20835816 | pubmed:pagination | 2125-32 | lld:pubmed |
| pubmed-article:20835816 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:20835816 | pubmed:meshHeading | pubmed-meshheading:20835816... | lld:pubmed |
| pubmed-article:20835816 | pubmed:meshHeading | pubmed-meshheading:20835816... | lld:pubmed |
| pubmed-article:20835816 | pubmed:meshHeading | pubmed-meshheading:20835816... | lld:pubmed |
| pubmed-article:20835816 | pubmed:meshHeading | pubmed-meshheading:20835816... | lld:pubmed |
| pubmed-article:20835816 | pubmed:meshHeading | pubmed-meshheading:20835816... | lld:pubmed |
| pubmed-article:20835816 | pubmed:meshHeading | pubmed-meshheading:20835816... | lld:pubmed |
| pubmed-article:20835816 | pubmed:meshHeading | pubmed-meshheading:20835816... | lld:pubmed |
| pubmed-article:20835816 | pubmed:meshHeading | pubmed-meshheading:20835816... | lld:pubmed |
| pubmed-article:20835816 | pubmed:meshHeading | pubmed-meshheading:20835816... | lld:pubmed |
| pubmed-article:20835816 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20835816 | pubmed:articleTitle | Electrochemiluminescent aptamer biosensor for the determination of ochratoxin A at a gold-nanoparticles-modified gold electrode using N-(aminobutyl)-N-ethylisoluminol as a luminescent label. | lld:pubmed |
| pubmed-article:20835816 | pubmed:affiliation | State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. wangzp1974@hotmail.com | lld:pubmed |
| pubmed-article:20835816 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20835816 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:20835816 | lld:pubmed |
