Source:http://linkedlifedata.com/resource/pubmed/id/20835816
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2010-10-20
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pubmed:abstractText |
A highly selective electrochemiluminescent biosensor for the detection of target nephrotoxic toxin, ochratoxin A (OTA), was developed using a DNA aptamer as the recognition element and N-(4-aminobutyl)-N-ethylisoluminol (ABEI) as the signal-producing compound. The electrochemiluminescent aptamer biosensor was fabricated by immobilizing aptamer complementary DNA 1 sequence onto the surface of a gold-nanoparticle (AuNP)-modified gold electrode. ABEI-labeled aptamer DNA 2 sequence hybridized to DNA 1 and was utilized as an electrochemiluminescent probe. A decreased electrochemiluminescence (ECL) signal was generated upon aptamer recognition of the target OTA, which induced the dissociation of DNA 2 (ABEI-labeled aptamer electrochemiluminescent probe) from DNA 1 and moved it far away from the electrode surface. Under the optimal conditions, the decreased ECL intensity was proportional to an OTA concentration ranging from 0.02 to 3.0 ng mL(-1), with a detection limit of 0.007 ng mL(-1). The relative standard deviation was 3.8% at 0.2 ng mL(-1) (n = 7). The proposed method has been applied to measure OTA in naturally contaminated wheat samples and validated by an official method. This work demonstrates the combination of a highly binding aptamer with a highly sensitive ECL technique to design an electrochemiluminescent biosensor, which is a very promising approach for the determination of small-molecule toxins.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/6-(N-(4-aminobutyl)-N-ethyl)amino-2...,
http://linkedlifedata.com/resource/pubmed/chemical/Aptamers, Nucleotide,
http://linkedlifedata.com/resource/pubmed/chemical/Gold,
http://linkedlifedata.com/resource/pubmed/chemical/Luminol,
http://linkedlifedata.com/resource/pubmed/chemical/Ochratoxins,
http://linkedlifedata.com/resource/pubmed/chemical/ochratoxin A
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1618-2650
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
398
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2125-32
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:20835816-Aptamers, Nucleotide,
pubmed-meshheading:20835816-Biosensing Techniques,
pubmed-meshheading:20835816-Electrodes,
pubmed-meshheading:20835816-Gold,
pubmed-meshheading:20835816-Luminescence,
pubmed-meshheading:20835816-Luminescent Measurements,
pubmed-meshheading:20835816-Luminol,
pubmed-meshheading:20835816-Metal Nanoparticles,
pubmed-meshheading:20835816-Ochratoxins
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pubmed:year |
2010
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pubmed:articleTitle |
Electrochemiluminescent aptamer biosensor for the determination of ochratoxin A at a gold-nanoparticles-modified gold electrode using N-(aminobutyl)-N-ethylisoluminol as a luminescent label.
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pubmed:affiliation |
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. wangzp1974@hotmail.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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