Source:http://linkedlifedata.com/resource/pubmed/id/20835428
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
23
|
pubmed:dateCreated |
2010-11-10
|
pubmed:abstractText |
Optoelectronic tweezers was used to manipulate human spermatozoa to determine whether their response to OET predicts sperm viability among non-motile sperm. We review the electro-physical basis for how live and dead human spermatozoa respond to OET. The maximal velocity that non-motile spermatozoa could be induced to move by attraction or repulsion to a moving OET field was measured. Viable sperm are attracted to OET fields and can be induced to move at an average maximal velocity of 8.8 ± 4.2 µm s(-1), while non-viable sperm are repelled to OET, and are induced to move at an average maximal velocity of -0.8 ± 1.0 µm s(-1). Manipulation of the sperm using OET does not appear to result in increased DNA fragmentation, making this a potential method by which to identify viable non-motile sperm for assisted reproductive technologies.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
1473-0197
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
7
|
pubmed:volume |
10
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
3213-7
|
pubmed:meshHeading |
pubmed-meshheading:20835428-Cryopreservation,
pubmed-meshheading:20835428-DNA Fragmentation,
pubmed-meshheading:20835428-Equipment Design,
pubmed-meshheading:20835428-Humans,
pubmed-meshheading:20835428-Male,
pubmed-meshheading:20835428-Optical Tweezers,
pubmed-meshheading:20835428-Reproductive Techniques, Assisted,
pubmed-meshheading:20835428-Sperm Count,
pubmed-meshheading:20835428-Sperm Injections, Intracytoplasmic,
pubmed-meshheading:20835428-Sperm Motility,
pubmed-meshheading:20835428-Sperm Retrieval,
pubmed-meshheading:20835428-Spermatozoa
|
pubmed:year |
2010
|
pubmed:articleTitle |
Motile and non-motile sperm diagnostic manipulation using optoelectronic tweezers.
|
pubmed:affiliation |
Department of Electrical Engineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA. aohta@hawaii.edu
|
pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
|