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pubmed-article:2083373pubmed:abstractTextPurified rat islets were dissociated into single-cell suspension with an EDTA-Trypsin treatment. During a stationary culture in vitro the islet cells reassociated forming aggregates (neoislets). Electron microscopy revealed that the aggregates consisted mostly of beta-cells and not numerous alpha-cells. They showed a good insulin-secreting capacity and were able to increase insulin release in response to glucose plus theophylline. The lack of passenger leukocytes makes the neoislets particularly suited for experimental and clinical transplantation.lld:pubmed
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pubmed-article:2083373pubmed:articleTitle[Aggregates of islet cells, their structure and insulin-producing activity during cultivation in vitro].lld:pubmed
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