Linked Life Data

20831918

Source:http://linkedlifedata.com/resource/pubmed/id/20831918

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2010-10-25
pubmed:abstractText
The blood vessels are one of the important target tissues for the mediators of inflammation and allergy; further cytokines affect them in a number of ways. We review the use of the isolated blood vessel mounted in organ baths as an important source of pharmacological information. While its use in the bioassay of vasoactive substances tends to be replaced with modern analytical techniques, contractility assays are effective to evaluate novel synthetic drugs, generating robust potency and selectivity data about agonists, partial agonists and competitive or insurmountable antagonists. For instance, the human umbilical vein has been used extensively to characterize ligands of the bradykinin B(2) receptors. Isolated vascular segments are live tissues that are intensely reactive, notably with the regulated expression of gene products relevant for inflammation (e.g., the kinin B(1) receptor and inducible nitric oxide synthase). Further, isolated vessels can be adapted as assays of unconventional proteins (cytokines such as interleukin-1, proteases of physiopathological importance, complement-derived anaphylatoxins and recombinant hemoglobin) and to the gene knockout technology. The well known cross-talks between different cell types, e.g., endothelium-muscle and nerve terminal-muscle, can be extended (smooth muscle cell interaction with resident or infiltrating leukocytes and tumor cells). Drug metabolism and distribution problems can be modeled in a useful manner using the organ bath technology, which, for all these reasons, opens a window on an intermediate level of complexity relative to cellular and molecular pharmacology on one hand, and in vivo studies on the other.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1878-1705
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1344-53
pubmed:meshHeading
pubmed-meshheading:20831918-Animals, pubmed-meshheading:20831918-Biological Assay, pubmed-meshheading:20831918-Blood Vessels, pubmed-meshheading:20831918-Cell Communication, pubmed-meshheading:20831918-Cytokines, pubmed-meshheading:20831918-Gene Expression, pubmed-meshheading:20831918-Humans, pubmed-meshheading:20831918-Inflammation Mediators, pubmed-meshheading:20831918-Male, pubmed-meshheading:20831918-Mice, pubmed-meshheading:20831918-Muscle, Smooth, Vascular, pubmed-meshheading:20831918-Muscle Contraction, pubmed-meshheading:20831918-Nitric Oxide Synthase Type II, pubmed-meshheading:20831918-Organ Culture Techniques, pubmed-meshheading:20831918-Peptide Hydrolases, pubmed-meshheading:20831918-Rats, pubmed-meshheading:20831918-Receptor, Bradykinin B2, pubmed-meshheading:20831918-Umbilical Veins
pubmed:year
2010
pubmed:articleTitle
Vascular smooth muscle contractility assays for inflammatory and immunological mediators.
pubmed:affiliation
Centre de recherche en rhumatologie et immunologie, Centre Hospitalier Universitaire de Québec, Québec, QC, Canada. francois.marceau@crchul.ulaval.ca
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't