Linked Life Data

20831345

Source:http://linkedlifedata.com/resource/pubmed/id/20831345

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-9-13
pubmed:abstractText
Agarose hydrogels containing aminopropyl triethoxy silane (APTS) have been prepared and evaluated as scaffolds for adhesion and proliferation of human mesenchymal stem cells (hMSCs). The preparation of the hydrogels involved the conventional melting of agarose in water followed by addition of APTS as functional group carrier. The resulting hydrogel supports have been studied by Fourier transformed infrared spectroscopy in order to get an insight into the hybrid molecular structure. X-ray photoelectron spectroscopy has been used for the analysis of the surface chemical composition of the hydrogels. It is deduced from these data that the resulting hybrid structure presents two phases with a clear tendency toward APTS surface segregation. Moreover, the observation of the desiccated hydrogel surfaces by atomic force microscopy shows that the films acquire a filament-mesh structure for increasing APTS content, while the pure agarose supports exhibit a granular structure. As a result of such a structure, the hydrogel surfaces show a hydrophobic behavior, as determined by water contact angle measurements. The biocompatibility of such platforms is supported by adhesion-proliferation assays performed with hMSCs. It is concluded that although adhesion is lower on APTS rich scaffolds, the proliferation rate on these surfaces is higher so that total number of proliferating cells does not significantly depend on APTS content in the hydrogels.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1559-4106
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
23-9
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Characterization and cytocompatibility of hybrid aminosilane-agarose hydrogel scaffolds.
pubmed:affiliation
Departamento de Física Aplicada and Departamento de Biología Molecular, Universidad Autónoma de Madrid and Centro de Investigaciones Biomédicas en Red, Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), 28049 Madrid, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't