Linked Life Data

20824694

Source:http://linkedlifedata.com/resource/pubmed/id/20824694

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-10-20
pubmed:abstractText
Bone remodeling and, therefore, integration of implant materials require the coordinated regulation of osteoblast and osteoclast activity. This is why the in vitro evaluation of biomaterials for bone regeneration should involve not only the analysis of osteoblast differentiation but also the formation and differentiation of osteoclasts. In the present study, we applied a material made of mineralized collagen I that mimics extracellular bone matrix to establish a culture system, which allows the cocultivation of human monocytes and human mesenchymal stem cells (hMSCs), which were differentiated into osteoclast-like cells and osteoblasts, respectively. Both cell types were cultivated on membrane-like structures from mineralized collagen. Transwell inserts were used to spatially separate the cell types but allowed exchange of soluble factors. The osteoclastogenesis and osteogenic differentiation were evaluated by analysis of gene expression, determination of alkaline phosphatase (ALP), and tartrate-resistant acidic phosphatase (TRAP) activity. Furthermore, cell morphology was studied using scanning electron and transmission electron microscopy. Osteogenically induced hMSC showed an increased specific ALP activity as well as increased gene expression of gene coding for alkaline phosphatase (ALPL), when cocultivated with differentiating osteoclasts. Adipogenic differentiation of hMSCs was suppressed by the presence of osteoclasts as indicated by a major decrease in adipocyte cell number and a decrease in gene expression of adipogenic markers. The formation of multinucleated osteoclasts seems to be decreased in the presence of osteogenically induced hMSC as indicated by electron microscopic evaluation and determination of TRAP activity. However, gene expression of osteoclast markers was not decreased in coculture with osteogenically induced hMSC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1552-4965
pubmed:author
pubmed:copyrightInfo
© 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
848-56
pubmed:meshHeading
pubmed-meshheading:20824694-Acid Phosphatase, pubmed-meshheading:20824694-Alkaline Phosphatase, pubmed-meshheading:20824694-Biocompatible Materials, pubmed-meshheading:20824694-Bone Remodeling, pubmed-meshheading:20824694-Cell Culture Techniques, pubmed-meshheading:20824694-Cell Differentiation, pubmed-meshheading:20824694-Cell Proliferation, pubmed-meshheading:20824694-Cells, Cultured, pubmed-meshheading:20824694-Coculture Techniques, pubmed-meshheading:20824694-Collagen, pubmed-meshheading:20824694-Extracellular Matrix, pubmed-meshheading:20824694-Humans, pubmed-meshheading:20824694-Isoenzymes, pubmed-meshheading:20824694-Materials Testing, pubmed-meshheading:20824694-Mesenchymal Stem Cells, pubmed-meshheading:20824694-Monocytes, pubmed-meshheading:20824694-Nanocomposites, pubmed-meshheading:20824694-Osteoblasts, pubmed-meshheading:20824694-Osteoclasts
pubmed:year
2010
pubmed:articleTitle
Crosstalk of osteoblast and osteoclast precursors on mineralized collagen--towards an in vitro model for bone remodeling.
pubmed:affiliation
Institute for Materials Science and Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Str 27, 01069 Dresden, Germany. abernhard@nano.tu-dresden.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies