Linked Life Data

20824627

Source:http://linkedlifedata.com/resource/pubmed/id/20824627

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2010-9-23
pubmed:abstractText
Well-characterized resins of high purity are critical for effective solid phase peptide synthesis (SPPS). The quality of commercial (4-methyl)benzhydrylamine-resin (MBHA-resin), used for the synthesis of peptide amides, is not consistent and residual ketone functionalities are frequently present. Such ketone or aldehyde impurities lead to the formation of acylation-resistant deletion peptides in SPPS. To avoid these undesirable side reactions, we have optimized the preparation of two amide-generating linkers, which, in combination with aminomethyl-resin prepared directly from polystyrene resin, serve as alternatives to MBHA-resin for peptide amide synthesis. Then we have explored their comparative properties in SPPS. Use of sonication in reductive amination facilitated the synthesis of both benzhydrylamine (BHA) and MBHA linkers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1099-1387
pubmed:author
pubmed:copyrightInfo
© 2010 European Peptide Society and John Wiley & Sons, Ltd.
pubmed:issnType
Electronic
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
545-50
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Synthesis and comparative properties of two amide-generating resin linkers for use in solid phase peptide synthesis.
pubmed:affiliation
Department of Chemistry, University of Chicago, Chicago, IL 60615, USA. dengprotein@uchicago.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.