Linked Life Data

20822308

Source:http://linkedlifedata.com/resource/pubmed/id/20822308

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-9-8
pubmed:databankReference
pubmed:abstractText
Pigs offer an unlimited source of xenografts for humans. However, vertically transmitted Porcine endogenous retrovirus (PERV) poses an infectious risk in the course of pig-to-human transplantation. In this study, we characterized PERV long terminal repeat (LTR) sequences from three species of Chinese pigs Banna minipig inbred (BMI), Wu-Zhi-Shan pig (WZSP), and Neijiang pig (NJP-A), and compared them with those of known PERVs (PERV-A, PERV-B, PERV-C, PERV-NIH, and 293-PERV-43). Genomic DNA extracted from peripheral blood mononuclear cells (PBMCs) of the Chinese pigs was used for PCR-amplification, cloning, and sequencing of LTRs. The sequences of BMI and WZSP LTRs were found identical with those of PERV-A and PERV-B, while that of NJP-A LTR was found close to those of PERV-C and PERV-NIH. The gammaretroviral nature of PERV LTRs from Chinese pigs was proved. These LTRs contained also promoter elements including enhancer-like repeats comparable with those of other PERVs. These findings suggested that PERVs from Chinese pigs were similar to PERV-A and PRV-B. Moreover, this study provided new data for the evaluation and selection of pigs to be used in the xenotransplantation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0001-723X
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
165-72
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Molecular characterization of long terminal repeat of porcine endogenous retroviruses in Chinese pigs.
pubmed:affiliation
Department of Urology, West China Hospital, Sichuan University, Chengdu, P.R. China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't