|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0003765,
umls-concept:C0007589,
umls-concept:C0012854,
umls-concept:C0017262,
umls-concept:C0024501,
umls-concept:C0030956,
umls-concept:C0086022,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0205349,
umls-concept:C0282641,
umls-concept:C0442335,
umls-concept:C0721534,
umls-concept:C1257975,
umls-concept:C1511938,
umls-concept:C1705099,
umls-concept:C1705822,
umls-concept:C2911684
|
|
pubmed:issue |
9
|
|
pubmed:dateCreated |
2010-9-7
|
|
pubmed:abstractText |
The ability of human mesenchymal stem cells (hMSCs) to differentiate into specific cells holds promise for therapeutic use in cell- or gene-based therapy. Genetic modification of hMSCs by introduction of therapeutic genes is an important tool for successful cell-mediated gene therapy. Similar to most primary cells, hMSCs are difficult to transfect with currently available gene delivery methods. Viral vectors are the most efficient DNA delivery system in stem cells. However, the use of viral vectors has disadvantages involving safety. To overcome these problems, nonviral gene delivery has been studied as an alternative strategy, and the cationic peptide was an efficient vector for transfecting various mammalian cell types. However, little is known about the capacity of this delivery method to transfect to hMSCs. In the present study, we examined the use of a short arginine peptide as a carrier for DNA delivery in hMSCs.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
1521-2254
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
12
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
779-89
|
|
pubmed:meshHeading |
pubmed-meshheading:20821748-Adipocytes,
pubmed-meshheading:20821748-Arginine,
pubmed-meshheading:20821748-Cell Differentiation,
pubmed-meshheading:20821748-Cell Survival,
pubmed-meshheading:20821748-Cells, Cultured,
pubmed-meshheading:20821748-DNA,
pubmed-meshheading:20821748-Drug Delivery Systems,
pubmed-meshheading:20821748-Erythropoietin,
pubmed-meshheading:20821748-Flow Cytometry,
pubmed-meshheading:20821748-Gene Transfer Techniques,
pubmed-meshheading:20821748-Genetic Vectors,
pubmed-meshheading:20821748-Green Fluorescent Proteins,
pubmed-meshheading:20821748-Humans,
pubmed-meshheading:20821748-Mesenchymal Stem Cells,
pubmed-meshheading:20821748-Microscopy, Atomic Force,
pubmed-meshheading:20821748-Osteoblasts,
pubmed-meshheading:20821748-Peptide Fragments,
pubmed-meshheading:20821748-Transfection,
pubmed-meshheading:20821748-Transgenes
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
An hydrophobically modified arginine peptide vector system effectively delivers DNA into human mesenchymal stem cells and maintains transgene expression with differentiation.
|
|
pubmed:affiliation |
Department of Life Science, Sogang University, Seoul, Republic of Korea.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
