Linked Life Data

20820491

Source:http://linkedlifedata.com/resource/pubmed/id/20820491

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2010-9-21
pubmed:abstractText
We have employed atomic force microscope-based infrared spectroscopy (AFM-IR) to spatially map energy storage polymers inside individual bacteria Rhodobacter capsulatus. AFM-IR allows chemical mapping of sub-cellular features with a spatial resolution of <100 nm. We have used key absorption bands of the energy storage polymer polyhydroxybutyrate (PHB) known from FTIR to spatially map the molecular distribution of PHB inside bacteria. We have also compared FTIR measurements on bulk PHB with AFM-IR measurements of PHB inside bacteria. We observe a shift in the location of the carbonyl absorption peak between bulk PHB and PHB inside bacteria. We have also used finite element analysis to model AFM-IR measurements of PHB granules, allowing for estimation of the real size of the granules. We have also performed transmission electron microscopy (TEM) of R. capsulatus to determine the size distribution of the PHB granules. Sizes measured by AFM-IR correspond well to TEM measurements.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1364-5528
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
135
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2540-5
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
In situ identification and imaging of bacterial polymer nanogranules by infrared nanospectroscopy.
pubmed:affiliation
Laboratoire de Chimie Physique, Université Paris-Sud, 91405 Orsay Cedex, France.
pubmed:publicationType
Journal Article