Source:http://linkedlifedata.com/resource/pubmed/id/20816679
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2010-10-5
|
| pubmed:abstractText |
Cofactor for acrosome reaction-inducing substance (Co-ARIS) is a steroidal saponin from the starfish Asterias amurensis. Saponins exist in many plants and few animals as self-defensive chemicals, but Co-ARIS has been identified as a cofactor for inducing the acrosome reaction (AR). In A. amurensis, the AR is induced by the cooperative action of egg coat components (ARIS, Co-ARIS, and asterosap); however, the mechanism of action of Co-ARIS is obscure. In this study we elucidated the membrane dynamics involved in the action of Co-ARIS. We found that cholesterol specifically inhibited the Co-ARIS activity for AR induction and detected the binding of labeled compounds with sperm using radioisotope-labeled Co-ARIS. Co-ARIS treatment did not reduce the content of sperm sterols, however, the condition was changed and localization of GM1 ganglioside on the periacrosomal region disappeared. We then developed a caveola-breaking assay, a novel method to detect the effect of chemicals on microdomains of culture cell, and confirmed the disturbance of somatic cell caveolae in the presence of Co-ARIS. Finally, by atomic force microscopy observations and surface plasmon resonance measurements using an artificial membrane, we revealed that Co-ARIS colocalized with GM1 clusters on the microdomains. Through this study, we revealed a capacitation-like event for AR in starfish sperm.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol,
http://linkedlifedata.com/resource/pubmed/chemical/G(M1) Ganglioside,
http://linkedlifedata.com/resource/pubmed/chemical/Membranes, Artificial,
http://linkedlifedata.com/resource/pubmed/chemical/Saponins,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
1095-564X
|
| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2010 Elsevier Inc. All rights reserved.
|
| pubmed:issnType |
Electronic
|
| pubmed:day |
1
|
| pubmed:volume |
347
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
147-53
|
| pubmed:meshHeading |
pubmed-meshheading:20816679-Acrosome Reaction,
pubmed-meshheading:20816679-Animals,
pubmed-meshheading:20816679-Asterias,
pubmed-meshheading:20816679-Binding Sites,
pubmed-meshheading:20816679-Biological Assay,
pubmed-meshheading:20816679-CHO Cells,
pubmed-meshheading:20816679-Caveolae,
pubmed-meshheading:20816679-Cholesterol,
pubmed-meshheading:20816679-Cricetinae,
pubmed-meshheading:20816679-Cricetulus,
pubmed-meshheading:20816679-G(M1) Ganglioside,
pubmed-meshheading:20816679-Kinetics,
pubmed-meshheading:20816679-Male,
pubmed-meshheading:20816679-Membrane Microdomains,
pubmed-meshheading:20816679-Membranes, Artificial,
pubmed-meshheading:20816679-Molecular Conformation,
pubmed-meshheading:20816679-Saponins,
pubmed-meshheading:20816679-Spermatozoa,
pubmed-meshheading:20816679-Tritium
|
| pubmed:year |
2010
|
| pubmed:articleTitle |
Acrosome reaction-related steroidal saponin, Co-ARIS, from the starfish induces structural changes in microdomains.
|
| pubmed:affiliation |
Department of Biosciences and Informatics, Keio University, Yokohama, Kanagawa, 223-8522, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|