Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-9-6
pubmed:abstractText
The genome is virtually identical in all cells within an organism, with epigenetic changes contributing largely to the plasticity in gene expression during both development and aging. These changes include covalent modifications of chromatin components and altered chromatin organization as well as changes in other nuclear components, such as nuclear envelope lamins. Given that DNA in each chromosome is centimeters long and dozens of chromosomes are compacted into a microns-diameter nucleus through non-trivial interactions with the bounding envelope, the polymer physics of such a structure under stress can be complex but perhaps systematic. We summarize micromanipulation methods for measuring the physical plasticity of the nucleus, with recent studies documenting the extreme flexibility of human embryonic stem cells and the rigidification in model aging of progerin-type nuclei. Lamin-A/C is a common molecular factor, and methods are presented for its knockdown and measurement.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-10912176, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-11373684, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-11784536, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-12725518, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-15635413, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-16005465, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-16055543, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-16179429, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-16645051, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-16849165, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-17360341, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-17673662, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-19158789, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-19815776, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-7251674, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-8106464, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-9486653, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-9600903, http://linkedlifedata.com/resource/pubmed/commentcorrection/20816236-9690471
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0091-679X
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Print
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
207-20
pubmed:dateRevised
2011-7-28
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Physical plasticity of the nucleus and its manipulation.
pubmed:affiliation
Biophysical Engineering Laboratory, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Review, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural